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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 05: 561 - 566

DOI: https://doi.org/10.6023/A1108112

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Preparation and Characterization of Temperature-responsive Affinity Chromatographic Stationary Phase Modified with Heparin

  • Liang Xiao ,
  • Liu Zongjian ,
  • Dai Rongji ,
  • Wang Wei ,
  • Geng Fangfang ,
  • Liang Yanli ,
  • Meng Weiwei ,
  • Deng Yulin
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  • School of Life Science, Beijing Institute of Technology, Beijing 100081

Received date: 2011-08-11

  Revised date: 2011-11-08

  Online published: 2012-03-17

Supported by

Project supported by the National Natural Science Foundation of China (No. 20675008) and the National Basic Research Program of China (973 Program, No. 2007CB914103).

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Abstract

The silica beads modified by poly(N-isopropylacrylamide-co-N-acryloxysuccinimide) and heparin, which was then used as temperature-responsive affinity chromatographic stationary phase were prepared via radical polymerization. Grafting ratio of polymers grafted on surfaces was 9.45% calculated by thermogravimetric analysis (TGA) and the heparin content on surfaces was 3.6 mg/g determined by Elson-Morgan method. The packing column with the modified silica beads as the HPLC stationary phase had temperature- responsive features, which was proved by separating benzene and hydrocortisone. The results also indicated that the strong affinity interaction occurs between thrombin and the modified column, with recovery of 81.4%. The releasing time of thrombin was shortened by controlling the column temperature, which provided a fast and efficient method for protein separation.

Key words： high-performance liquid chromatography; poly(N-isopropylacrylamide); heparin; thrombin

Cite this article

Liang Xiao , Liu Zongjian , Dai Rongji , Wang Wei , Geng Fangfang , Liang Yanli , Meng Weiwei , Deng Yulin . Preparation and Characterization of Temperature-responsive Affinity Chromatographic Stationary Phase Modified with Heparin[J]. Acta Chimica Sinica, 2012 , 70(05) : 561 -566 . DOI: 10.6023/A1108112

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