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Study on the Influence of Rhizoma Zingiberis on the Absorptive Profile of Aconite Alkaloids in the Rat Gut Sacs by Ultra Performance Liquid Chromatography-Mass Spectrometry

  • Xin Yang ,
  • Pi Zifeng ,
  • Song Fengrui ,
  • Liu Zhiqiang ,
  • Liu Shuying
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  • a Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022;
    b Graduate School of the Chinese Academy of Sciences, Beijing 100039

Received date: 2011-08-31

  Revised date: 2011-11-08

  Online published: 2012-03-17

Supported by

Project supported by the National Natural Science Foundation of China (Nos. 81073040, 30472134, 21175127), the National Basic Research Program of China (“973 Program”) (No. 2011CB505300-05) and Jilin Province Science and Technology Department (No. YYZX201131).

Abstract

Everted rat gut sac model was used to carry out the experiments about detoxification mechanism of Rhizoma Zingiberis on the diester-aconite alkaloids in Radix Aconiti. All the diester-aconite alkaloids were detected by Waters Ultra Performance Liquid Chromatography coupled with triple quadrupole Mass Spectrometry. Content of aconitine, mesaconitine and hypaconitine were calculated by standard curve method and other diester-aconite alkaloids were calculated by peak area. Results showed that the accumulative absorptive content of aconitine, mesaconitine, hypaconitine and the peak area of 10-OH-mesaconitine in unit area of the rat gut sac decreased when the extract of Rhizoma Zingiberis was added into K-R solution contained the exact of Radix Aconiti, and that increased when verapamil was added into K-R solution contained the exact of Radix Aconiti, which indicated the diester-aconite alkaloids might be the potential substrate of P-glycoprotein (P-gp). The accumulative content of digoxin in unit area of the rat gut sac decreased when the extract of Rhizoma Zingiberis was added into the intestinal nutritious solution contained digoxin, which indicated the extract of Rhizoma Zingiberis might be the revulsant of P-glycoprotein. On the basis of the above results, it could be concluded that the possible mechanism of the inhibition of the extract of Rhizoma Zingiberis on the absorption of the aconite alkaloids in the rat gut sacs was the extract of Rhizoma Zingiberis which served as the potential revulsant could inhibit the absorption of aconite alkaloids by inhibiting the activity of the intestinal P-gp.

Cite this article

Xin Yang , Pi Zifeng , Song Fengrui , Liu Zhiqiang , Liu Shuying . Study on the Influence of Rhizoma Zingiberis on the Absorptive Profile of Aconite Alkaloids in the Rat Gut Sacs by Ultra Performance Liquid Chromatography-Mass Spectrometry[J]. Acta Chimica Sinica, 2012 , 70(05) : 551 -560 . DOI: 10.6023/A1108311

References

1 Rao, C. L.; Peng, C. J. Toxicol. 2010, 24, 94 (in Chinese). (饶朝龙, 彭成, 毒理学杂志, 2010, 24, 94.)

2 Wang, H. L.; Dong, L.; Xu, D. F.; Zhang, X. J. J. Liaoning Univ. Trad. Chin. Med. 2010, 12, 191 (in Chinese). (王海龙, 董雷, 许大方, 张学金, 辽宁中医药大学学报,2010, 12, 191.)

3 Bao, J.; Wang, J. N.; Zhang, C. B. J. Shandong Univ. Trad. Chin. Med. 2011, 35, 108 (in Chinese). (鲍捷, 王均宁, 张成博, 山东中医药大学学报, 2011, 35,108.)

4 Xu, S. J.; Chen, C. X.; Gao, J. P. Lishizhen Med. Mater. Med. Res. 2006, 17, 518 (in Chinese). (徐姗君, 陈长勋, 高建平, 时珍国医国药, 2006, 17, 518.)

5 Zhang, Y. F.; Li, Y. J.; Yang, Q.; Weng, X. G.; Dong, Y.; Zhu, X. X. Chin. J. Exper. Trad. Med. Formulae 2010, 16, 107 (in Chinese). (张英丰, 李玉洁, 杨庆, 翁小刚, 董宇, 朱晓新, 中国实 验方剂学杂志, 2010, 16, 107.)

6 Bi, X. L.; Cheng, J. Chin. JMAP, 2010, 27, 92 (in Chinese). (毕肖林, 程锦, 中国现代应用药学, 2010, 27, 92.)

7 Ho, Y. F.; Lai, M. Y.; Yu, H. Y.; Huang, D. K.; Hsueh, W. C.; Tsai, T. H.; Lin, C. C. J. Formosan Med. Assoc. 2008,107, 37.  

8 Tian, X. J.; Yang, X. W.; Yang, X.; Wang, K. Int. J. Pharm.2009, 367, 58.

9 Lv, X. Y.; Bai, C. Y.; Chen, J. J.; Wang, Y. J.; Meng, Z. J.; Li, O.; Wang, C. Y.; Chen, L.; He, S. M. Chin. J. Gerontol.2010, 30, 2472 (in Chinese). (吕晓艳, 白彩艳, 陈加俊, 王云晶, 孟昭杰, 李鸥, 王春 艳, 陈立, 何淑梅, 中国老年学杂志, 2010, 30, 2472.)

10 Chan, K.; Liu, Z. Q.; Jiang, Z. H.; Zhou, H.; Wong, Y. F.; Xu, H. X.; Liu, L. J. Ethnopharmacol. 2006, 103, 425.  

11 Hui, Q. S. Academic Period. Farm Prod. Process. 2011, 7, 89 (in Chinese). (惠秋沙, 农产品加工, 2011, 7, 89.)

12 Xia, Y. G.; Liang, J.; Yang, B. Y.; Wang, Q. H.; Kuang, H. X. Information of TCM 2011, 28, 33 (in Chinese). (夏永刚, 梁军, 杨炳友, 王秋红, 匡海学, 中医药信息,2011, 28, 33.)

13 Verschraagen, M.; Koks, C. H. W.; Schellens, J. H. M.; Beijnen, J. H. Pharmacol. Res. 1999, 40, 301.  

14 Haslam, I. S.; Jones, K.; Coleman, T.; Simmons, N. L. Biochem. Pharmacol. 2008, 76, 850.  

15 Bansal, T.; Mishra, G.; Jaggi, M.; Khar, R. K.; Talegaonkar, S. Eur. J. Pharm. Sci. 2009, 36, 580.

16 Bai, X. Y. J. Prac. Trad. Chin. Med. 2005, 21, 125 (in Chinese). (白向阳, 实用中医药杂志, 2005, 21, 125.)
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