Via the coupling effect of γ-aminopropyltrimethoxysilane, methacrylic acid was graft-polymerized onto the surface of silica gel particles, obtaining the grafted particles PMAA/SiO₂. Then, the molecular imprinting towards the grafted PMAA was performed with one enantiomer of tetramisole (TM), L-TM, as template molecule and ethylene glycol diglycidyl ether (EGDE) as crosslinking agent by using the novel surface-molecular imprinting technique established by our research group, and the L-TM molecule-imprinted material MIP-PMAA/SiO₂ was prepared. With another enantiomer, D-TM, as a contrast compound, both static and dynamic methods were adopted to study the chiral recognition character and binding property of MIP-PMAA/SiO₂ for L-TM and to examine its ability to separate the two enantiomers in racemate. The experiment results show that MIP-PMAA/SiO₂ have specific recognition selectivity and excellent binding affinity for L-TM, and its selectivity coefficient for L-TM with respect to D-TM reaches 4.03, displaying fine ability to separate the two enantiomers. Besides, MIP-PMAA/SiO₂ has excellent elution property, and with a diluted aqueous solution of NaOH as the eluent, the desorption ratio of L-TM reaches 99.08% in 20 BV.