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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 01: 58 - 64

DOI: https://doi.org/10.6023/A1106072

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Fluorescence Quenching Method for the Determination of Analgin and Metabolin with Some Aromatic Amino Acids as Probes

  • GAN Xiao-Juan ,
  • LIU Shao-Pu ,
  • LIU Zhong-Fang ,
  • WANG Ya-Qiong ,
  • CUI Zhi-Ping ,
  • HU Xiao-Li
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  • Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715

Received date: 2011-06-07

  Revised date: 2011-07-31

  Online published: 2012-02-25

Supported by

Project supported by the National Natural Science Foundation of China(No.20875078).

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Abstract

In pH 3.2 Britton-Robinson(BR)buffer medium,analgin(ANG)and metabolin of 4-methylaminoantipyrine(MAA)and 4-acetylaminoantipyrine(AAA)can react with some aromatic amino acids such as tryptophane(Trp),tyrosine(Tyr)and phenylalanine(Phe)to form complexes,which lead to fluorescence quenching of above amino acids,and the maximum quenching wavelength is at 352 nm (ANG-Trp system),304 nm(ANG-Tyr,MAA-Tyr and AAA-Tyr system),284 nm(ANG-Phe system),respectively.Fluorescence quenching value(ΔF)is proportional to the concentration of ANG,MAA and AAA in a certain range.Fluorescence quenching methods for the determination of ANG,MAA and AAA have high sensitivity with the detection limits of 13.3 ng/mL(Trp-ANG system),15.8 ng/mL(Tyr-ANG system), 64.5 ng/mL(Phe-ANG system),150.0 ng/mL(MAA-Tyr system)and 230.8 ng/mL(AAA-Tyr system),re- spectively.The optimum conditions of the reaction,influencing factors and the effect of coexisting sub-stances are investigated,and the results show this method has a good selectivity.This method has been successfully applied to the quick determination of ANG in tablets and its metabolin in urine samples.Judging from the change of the absorption spectra,the effect of temperature and the Stern-Volmer plots,the quenching of fluorescence of amino acids by ANG is a static quenching process.Experimental results show that a 1:1 compound of ANG and amino acid is formed by electrostatic attraction and aromatic stacking interaction.

Key words： analgin; amino acids; 4-methylaminoantipyrine; 4-acetylaminoantipyrine; fluorescence quenching

Cite this article

GAN Xiao-Juan , LIU Shao-Pu , LIU Zhong-Fang , WANG Ya-Qiong , CUI Zhi-Ping , HU Xiao-Li . Fluorescence Quenching Method for the Determination of Analgin and Metabolin with Some Aromatic Amino Acids as Probes[J]. Acta Chimica Sinica, 2012 , 70(01) : 58 -64 . DOI: 10.6023/A1106072

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