Full Papers

Sterically Stabilized,Surface-Enhanced Raman Scattering-Tagged Gold Nanorod Probes for Immunoassay

  • LU Jun-Jun ,
  • GUO Hong-Yan ,
  • WANG Wei-Yu ,
  • PAN Jian-Gao ,
  • HU Jia-Wen
Expand
  • a. State Key Laboratory for Chemo/Biosensing and Chemometrics and College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082;
    b. Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei 230031

Received date: 2011-05-31

  Revised date: 2011-08-16

  Online published: 2012-02-25

Supported by

Project supported by the National Natural Science Foundation of China(Nos.20603008,20873037,and J0830415)and Open Project of State Key Laboratory of Supramolecular Structure and Materials(Jilin University)(No.SKLSSM200902).

Abstract

The immunoassay application of sterically stabilized,surface-enhanced Raman scattering (SERS)-tagged Au nanorod(NR)probes was reported.The probes were prepared by coadsorbing Raman-active molecule 4-mercaptobenzoic acid and biocompatible α-mercapto-ω-carboxyl polyethylene glycol onto the surfaces of Au NRs.The long PEG chain provides both protection and steric stabilization to the probes so that they are able to survive in adverse condition;meanwhile,its terminal carboxyl group binds target entity such as antibody,which imparts detection/recognition functionality to the probes.When the probe recognizes antigen(through the classical"sandwich"structure that was formed due to the highly binding specificity among capture antibody on a solid substrate,detected antigen,and antibody on the probe),the recognition is signaled by the SERS signals of the 4-mercaptobenzoic acid.The detection limit of the Au NR probes,for a single component antigen,was as low as 1×10-9mg·mL-1.

Cite this article

LU Jun-Jun , GUO Hong-Yan , WANG Wei-Yu , PAN Jian-Gao , HU Jia-Wen . Sterically Stabilized,Surface-Enhanced Raman Scattering-Tagged Gold Nanorod Probes for Immunoassay[J]. Acta Chimica Sinica, 2012 , 70(01) : 65 -70 . DOI: 10.6023/A1105311

References

1 Gutcho, S. ; Mansbach, L. Clin. Chem. 1977, 23, 1609.

2 Vuori, J. ; Rasi, S. ; Takala, T. ; Vaananen, K. Clin. Chem. 1991, 37, 2087.

3 Brown, C. R. ; Higgins, K. W. ; Frazer, K. ; Schoelz, L. K. ; Dyminski, J. W. ; Marinkovich, V. A. ; Miller, S. P. ; Burd, J. F. Clin. Chem. 1985, 31, 1500.

4 Hayes, F. J. ; Halsall, H. B. ; Heineman, W. R. Anal. Chem. 1994, 66, 1860.   

5 Butler, J. E. J. Immunoassay 2000, 21, 165.   

6 Sokolow, K. ; Byramova, N. E. ; Mochalova, L. V. Appl. Spectrosc. 1993, 47, 535.   

7 Mulvaney, S. P. ; Musick, M. D. ; Keating, C. D. ; Natan, M. J. Langmuir 2003, 19, 4784.   

8 Han, X. -X. ; Chen, L. ; Guo, J. ; Zhao, B. ; Ozaki, Y. Anal. Chem. 2010, 82, 4102.   

9 Wang, G. ; Park, H. -Y. ; Lipert, R. J. Anal. Chem. 2009, 81, 9643.   

10 Bao, F. ; Yao, J. -L. ; Gu, R. -A. Langmuir 2009, 25, 10782.   

11 Guo, H. -Y. ; Lu, L. -H. ; Wu, C. ; Pan, J. -G. ; Hu, J. -W. Acta Chim. Sinica 2009, 67(14), 1603(in Chinese). (郭红燕, 芦玲慧, 吴超, 潘建高, 胡家文, 化学学报, 2009, 67(14), 1603. )

12 Hwang, H. ; Chon, H. ; Choo, J. ; Park, J. -K. Anal. Chem. 2010, 82, 7603.   

13 Chon, H. ; Lim, C. ; Ha, S. -M. ; Ahn, Y. ; Lee, E. K. ; Chang, S. -I. ; Seong, G. H. ; Choo, J. Anal. Chem. 2010, 82, 5290.   

14 Ni, J. ; Lipert, R. J. ; Dawson, G. B. ; Porter, M. D. Anal. Chem. 1999, 71, 4903.   

15 Charles, C. Y. ; Jin, R. ; Mirkin, C. A. Science 2002, 297, 1536.   

16 Basiruddin, S. K. ; Saha, A. ; Pradhan, N. ; Jana, N. R. J. Phys. Chem. C 2010, 114, 11009.   

17 Selvan, S. T. ; Tan, T. T. Y. ; Yi, D. K. ; Jana, N. R. Langmuir 2010, 26, 11631.   

18 Doering, W. E. ; Nie, S. Anal. Chem. 2003, 75, 6171.   

19 Qian, X. ; Peng, X. -H. ; Ansaril, D. O. ; Yin-Goen, Q. ; Chen, G. Z. ; Shin, D. M. ; Yang, L. ; Yong, A. N. ; Wang, M. D. ; Nie, S. Nat. Biotechnol. 2008, 26, 83.   

20 Huang, Y. ; Swarup, V. P. ; Bishnoi, S. W. Nano Lett. 2009, 9, 2914.   

21 Wu, C. ; Guo, H. -Y. ; Hu, J. -W. Acta Chim. Sinica 2009, 67(14), 1621(in Chinese). (吴超, 郭红燕, 胡家文, 化学学报, 2009, 67(14), 1621. )

22 Moghimi, S. M. ; Hunter, A. C. ; Murray, J. C. Pharmacol. Rev. 2001, 53, 283.

23 Xu, H. ; K?ll, M. Top. Appl. Phys. 2006, 103, 87.   

24 Kim, K. ; Lee, M. Y. ; Lee, H. B. ; Shin, K. S. Appl. Mater. Interfaces 2009, 1(10), 2174.

25 Chon, H. ; Lee, S. ; Son, S. K. ; Oh, C. W. ; Choo, J. Anal. Chem. 2009, 81, 3029.   

26 Guo, H. ; Ruan, F. ; Lu, L. ; Hu, J. ; Pan, J. ; Yang, Z. ; Ren, B. J. Phys. Chem. C 2009, 113, 10459.

27 Nikoobakht, B. ; El-Sayed, M. A. Chem. Mater. 2003, 15, 1957.   

28 Chang, S. -C. ; Chao, I. ; Tao, Y. -T. J. Am. Chem. Soc. 1994, 116, 6792.   

29 Hermanson, G. T. In Bioconjugate Techniques, Ed. :Her- manson, G. T. , Academic Press, San Diego, 1996, p. 169.   
Outlines

/