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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 03: 277 - 283

DOI: https://doi.org/10.6023/A1103022

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Inhibitory Effect of Both Nano Eu2O3 and Eu3+ on Proliferation of HepG2 Cells

  • LIU Jie ,
  • WEI Chun-Ying ,
  • YANG Pin
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  • Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006

Received date: 2011-03-02

  Revised date: 2011-09-27

  Online published: 2011-11-01

Supported by

Project supported by the National Natural Science Foundation of China (Nos. 21041008, 21171108), Shanxi Provincial Fund for Natural Sciences (No. 2009011012-2) and the Research Project Supported by Shanxi Scholarship Council of China (No. 2011-016).

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Abstract

The biological effects of rare earth elements and rare earth nanoparticles have aroused an extensive interest during the past few years. In vitro culture conditions, we reported the impact of nano Eu2O3 and Eu3+ on the HepG2 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flow cytometry and laser scanning confocal microscopy. The results suggested that the nano Eu2O3 at low concentrations does not make an obvious impact on cell growth whereas it can suspend cell division, and the percentage of cells in G2/M phase increase gradually and lead to necrosis at higher concentrations (≥600 μg·mL-1). In addition, nano Eu2O3 can be internalized by live HepG2 cells. Eu3+ at low concentrations (≤100 μmol·L-1) can slightly inhibit the growth of HepG2 cells by arresting the ratio of G0/G1 phase cells and triggerring apoptosis.

Key words： nano Eu2O3; Eu3+; HepG2 cells; apoptosis; rare earth

Cite this article

LIU Jie , WEI Chun-Ying , YANG Pin . Inhibitory Effect of Both Nano Eu2O3 and Eu3+ on Proliferation of HepG2 Cells[J]. Acta Chimica Sinica, 2012 , 70(03) : 277 -283 . DOI: 10.6023/A1103022

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