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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 03: 284 - 290

DOI: https://doi.org/10.6023/A1109143

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UPLC/Q-TOF-MS Based Chemical Profiling Approach to Evaluate Chemical Composition of Augmentation Toxicity in Combination of Radix Aconiti and Pinellia Praeparata

  • ZHOU Si-Si ,
  • MA Zeng-Chun ,
  • LIANG Qian-De ,
  • WANG Yu-Guang ,
  • TAN Hong-Ling ,
  • XIAO Cheng-Rong ,
  • ZHANG Bo-Li ,
  • GAO Yue
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  • a Anhui Medical University, Hefei 230032;
    b Institute of Radiation Medicine Sciences, Academy of Military Medical Sciences, Beijing 100850;
    c Tianjin University of Traditional Chinese Medicine, Tianjin 300193

Received date: 2011-09-14

  Revised date: 2011-11-08

  Online published: 2011-11-30

Supported by

This work was supported by the National Basic Research Program of China (Nos. 2012CB518402, 2011CB505304) and the Natural Science Foundation of Beijing (No. 7112110).

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Abstract

In the present UPLC/Q-TOF-MS study, an ultra performance liquid chromatography coupled with time-of-fight mass spectrometry (UPLC/Q-TOFMS) based chemical profiling approach was used to evaluate chemical constitution between co-decoction and mixed decoction of radix aconiti and pinellia praeparata. Two different kinds of decoctions, namely radix aconiti and pinellia praeparata co-decoction: water extract of mixed two herbs, and radix aconiti and pinellia praeparata mixed decoction: mixed water extract of each individual herbs, were prepared. Batches of these two kinds of decoction samples were subjected to UPLC/Q-TOF-MS analysis, the datasets of tR-m/z pairs, ion intensities and sample codes were processed with orthogonal partial least squared discriminant analysis (OPLS-DA) to holistically compare the difference between these two kinds of decoction samples. The results of positive ion mode showed significant difference between two kinds of decoction samples, the content of hypaconitine, mesaconitine, aconitine, deoxyaconitine, 10-OH-mesaconitine, 10-OH-aconitine increased, while the content of mesaconine, dehydrated benzoylmesaconine, dehydrated benzoylhypaconine, benzoylaconine, benzoylhypaconine, 10- OH-benzoylmesaconine decreased in samples of co-decoction of radix aconiti and pinellia praeparata. The content of diester-diterpenoid alkaloids increased, while the content of monoester-diterpenoid alkaloids decreased, which may be basis of chemical composition in combination of radix aconiti and pinellia praeparata.

Key words： radix aconiti; pinellia praeparata; compatibility; LC-MS

Cite this article

ZHOU Si-Si , MA Zeng-Chun , LIANG Qian-De , WANG Yu-Guang , TAN Hong-Ling , XIAO Cheng-Rong , ZHANG Bo-Li , GAO Yue . UPLC/Q-TOF-MS Based Chemical Profiling Approach to Evaluate Chemical Composition of Augmentation Toxicity in Combination of Radix Aconiti and Pinellia Praeparata[J]. Acta Chimica Sinica, 2012 , 70(03) : 284 -290 . DOI: 10.6023/A1109143

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