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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 03: 352 - 356

DOI: https://doi.org/10.6023/A1104252

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Investigations on the Enhanced Fluorescence of Sybr Green I by Coralyne-induced Double-stranded Structure of Adenine Oligonucleotides and a Sensitive and Selective Spectroflurometry of Coralyne

  • ZHOU Ya-Wen ,
  • LI Chun-Mei ,
  • XIONG Zu-Hong ,
  • HUANG Cheng-Zhi
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  • a Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715;
    b College of Physics and Technology, Southwest University, Chongqing 400715;
    c College of Pharmaceutical Sciences, Southwest University, Chongqing 400716

Received date: 2011-04-25

  Revised date: 2011-09-27

  Online published: 2011-10-25

Supported by

Project was supported by the National Creating New Drug Program of China (No. 2010ZX09401-306-1-4), National Natural Science Foundation of China (No. 21035005) and Scientific Research Foundation of Southwest University (No. XDJK2009A001).

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Abstract

Coralyne is one of the protoberberine alkaloids analogues. It was found that coralyne could induce adenine oligonucleotides (poly A16) to form a double strand DNA under quasi-physiological conditions, greatly enhancing the fluorescence of Sybr Green I (SG I), a DNA intercalative dye. With the enhanced fluorescence signals of SG I, a new method for the determination of coralyne has been developed in linear range of 60～600 nmol/L, with the detection limit (3σ) of 30 nmol/L. This proposed method is highly selective and other analogues have no interfering effects on the determination, which has been successfully applied for analysis of synthetic samples with the recovery from 92.5% to 105%, and the RSD lower than 3.1%.

Key words： coralyne; adenine oligonucleotides; Sybr Green I; fluorescence spectrometry

Cite this article

ZHOU Ya-Wen , LI Chun-Mei , XIONG Zu-Hong , HUANG Cheng-Zhi . Investigations on the Enhanced Fluorescence of Sybr Green I by Coralyne-induced Double-stranded Structure of Adenine Oligonucleotides and a Sensitive and Selective Spectroflurometry of Coralyne[J]. Acta Chimica Sinica, 2012 , 70(03) : 352 -356 . DOI: 10.6023/A1104252

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