SIOC 中文
ACS
Adv search

Acta Chimica Sinica >

2012 , Vol. 0 >Issue 05: 599 - 605

DOI: https://doi.org/10.6023/A1012284

Full Papers

Thermodynamic Study on the Monolayer Mixed with Different Chol-content and PE/PC and the Morphology Observation of the Bilayers

  • Wang Juan ,
  • Chang Yiguang ,
  • Sun Runguang
Expand
  • Laboratory of Biophysics and Biomedicine, College of Physics and Information Technology, Shaanxi Normal University, Xi'an, 710062

Received date: 2010-12-28

  Revised date: 2011-10-10

  Online published: 2012-03-17

Supported by

Project supported by the National Natural Science Foundation of China (No. 20772077), and Research Fund for the Doctoral Program of Higher Education of China (No. 200807180001).

Fold

Abstract

The effect of different cholesterol (Chol)-content on the mixed monolayer of the phosphatidylethanolamine (PE)/phosphatidylcholine (PC) (1∶1 molar ratio) has been investigated by analyzing the surface pressure-area isotherms. And the thermodynamic behavior of mixed monolayer of Chol and PE/PC (1∶1 molar ratio) has been investigated by calculating and analyzing the excess molecular area and excess Gibbs energy (ΔAex and ΔGex). The results show that Chol accelerated the phase transition and promoted the aggregation of monolayer. When XChol＝0.2, 0.6, 0.8, ΔAex and ΔGex displayed negative values, suggesting attractive interaction between molecules. While XChol＝0.4, ΔAex and ΔGex displayed positive values at the surface pressure of 15, 20, 25, 30 mN/m, indicating repelling interaction between molecules, promoting the increasing of the entropy. In addition, the deposited Langmuir-Blodgett (LB) monolayers of different ratios were investigated with atomic force microscopy (AFM).

Key words： biomembrane; the LB film technique; excess Gibbs energy; isotherm; atomic force microscopy

Cite this article

Wang Juan , Chang Yiguang , Sun Runguang . Thermodynamic Study on the Monolayer Mixed with Different Chol-content and PE/PC and the Morphology Observation of the Bilayers[J]. Acta Chimica Sinica, 2012 , 0(05) : 599 -605 . DOI: 10.6023/A1012284

References

1 Liu, Y.-H.; Hu, L.; Xu, F.; Liu, S.; Wang, W.-B. Chin. J. Med. Phys. 2008, 25, 916 (in Chinese). (刘艳辉, 胡林, 许锋, 刘杉, 王文波, 中国医学物理学杂 志, 2008, 25, 916.)

2 Hao, C.-C. M.S. Thesis, Shaanxi Normal University, Xi'an, 2007 (in Chinese). (郝长春, 硕士论文, 陕西师范大学, 西安, 2007.)

3 Longo, M. L.; Blanchette, C. D. Biochim. Biophys. Acta-Biomembanes 2010, 1798, 1357.  

4 Mercado, F. V.; Maggio, B.; Wilke, N. Chem. Phys. Lipids2011, 164, 386.  

5 Marques, J. T.; Viana, A. S.; De Almeida, R. F. M. Biochim. Biophys. Acta-Biomembanes 2011, 1808, 405.  

6 Sui, S.-F. Molecular Membrane Biology, Higher Education Press, Beijing, 2003, pp. 7～428 (in Chinese). (隋森芳, 膜分子生物学, 高等教育出版社, 北京, 2003, pp. 7～428.)

7 Yang, F.-Y. Biomembrane, Science Press, Beijing, 2005, pp. 382～387 (in Chinese). (杨福愉, 生物膜, 科学出版社, 北京, 2005, pp. 382～387.)

8 Kirat, K. E.；Morandat, S.; Dufrene, Y. F. Biochim. Biophys. Acta 2010, 1798, 750.  

9 Sun, R.-G.; Zhang, J. Atom. Mol. Phys. 2003, 20, 85 (in Chinese). (孙润广, 张静, 原子与分子物理学报, 2003, 20, 85.)

10 Sun, R.-G.; Zhang, J.; Wang, Y.-C. Prog. Biochem. Biophys. 2000, 27, 296 (in Chinese). (孙润广, 张静, 王永昌, 生物化学与生物物理进展,2000, 27, 296.)

11 Chen, Y. Y.; Sun, R. G.; Wang, B. J. Colloid Interface Sci.2011, 353, 294.  

12 Garcia-Manyes, S.; Domenech, O.; Sanz, F.; Montero, M. F.; Hemandez-Borrell, J. Biochim. Biophys. Acta 2007,1768, 1191.

13 Deleu, M.; Nott, K.; Brasseur, R.; Jacques, P.; Thonart, P.; Dufrene, Y. F. Biochim. Biophys. Acta 2001, 1513, 55.  

14 He, P.-S. Polymerization in Two-dimensional State Polymerization in Monolayer and Langmuir-Blodgett Film, Press of University of Science and Technology of China, Hefei,2008, pp. 6～53 (in Chinese). ( 何平笙, 二维状态下的聚合- 单分子膜和 Langmuir-Blodgett 膜的聚合, 中国科学技术大学出版 社, 合肥, 2008, pp. 6～53.)  

15 Yang, X.-L.; Sun, R.-G.; Zhang, J. Chin. J. Liquid Crystals Displays 2006, 21, 349 (in Chinese). (杨小乐, 孙润广, 张静, 液晶与显示, 2006, 21, 349.)

16 Hao, C.-C; Sun, R.-G. Atom. Mol. Phys. 2007, 24, 932 (in Chinese). (郝长春, 孙润广, 原子与分子物理学报, 2007, 24, 932.)

17 Serfis, A. B.; Brancato, S.; Fiesler, S. J. Biochim. Biophys. Acta 2001, 1511, 342.

18 Nichols-Smith, S.;The, S. Y.; Kuhl, T. L. Biochim. Biophys. Acta 2004, 1663, 82.  

19 Domenech, O.; Sanz, F.; Montero, M. F.; Hemandez- Borrell, J. Biochim. Biophys. Acta 2006, 1758, 213.  

20 Sun, R.-G.; Hao, C.-C.; Chang, Y.-G.; Zhang, J.; Niu, C.-L. Acta Chim. Sinica 2009, 67, 1808 (in Chinese). (孙润广, 郝长春, 常怡光, 张静, 牛春玲, 化学学报,2009, 67, 1808.)

21 Chang, Y.-G.; Sun, R.-G.; Hao, C.-C. Chem. J. Chin. Univ.2010, 31, 559 (in Chinese). (常怡光, 孙润广, 郝长春, 高等学校化学学报, 2010, 31,559.)
Options
Outlines

/

〈 〉