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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 11: 1263 - 1270

DOI: https://doi.org/10.6023/A1109192

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Effect of Non-ideality of Intracellular Solution on the Optimal Protocols for Cell Cryopreservation

  • LI Wei-Bing ,
  • QIAO He-Yuan ,
  • ZHAO Gang
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  • University of Science and Technology of China, Department of Electronic Science and Technology, Laboratory of Cryo-Biomedical Engineering, Hefei 230027, Anhui Province

Received date: 2011-09-19

  Revised date: 2012-03-30

  Online published: 2012-03-30

Supported by

Supported by the National Natural Science Foundation of China (No. 51076149) and the Fundamental Research Funds for the Central Universities.

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Abstract

To predict the optimal cryopreservation protocol for biological cells, a new intracellular ice formation model was developed by incorporating the nonideality into the intracellular solution. Compared with the ideal solution model based on the modified Mazur’s water transport equation, the new model further coupled Fahy’s water transport equation, ice nucleation, and the diffusion-limited ice growth theory. The new model was then used to study the freezing process of mouse oocyte in the presence of glycerol as a cryoprotective agent. Through the comparative analysis of the influence of cooling rate and initial glycerol concentration on the intracellular solution volume and the crystallized volume fraction under these two models, it was found that the predictions by these two models had significant difference at low cooling rate, while they are consistent at high cooling rate.

Key words： intracellular ice formation; vitrification; nonideal solution

Cite this article

LI Wei-Bing , QIAO He-Yuan , ZHAO Gang . Effect of Non-ideality of Intracellular Solution on the Optimal Protocols for Cell Cryopreservation[J]. Acta Chimica Sinica, 2012 , 70(11) : 1263 -1270 . DOI: 10.6023/A1109192

References

1 Hua, T.-C.; Ren, H.-S. Cryobiomedical Techniques, Science Press, Beijing, 1984 (in Chinese). (华泽钊, 任禾盛, 低温生物医学技术, 科学出版社, 北 京, 1994.)  

2 Zhao, G. Ph.D. Dissertation, University of Science and Technology of China, Hefei, 2004 (in Chinese). (赵刚, 博士论文, 中国科学技术大学, 合肥, 2004.)  

3 Mazur, P.; Leibo, S. P.; Chu, E. H. Y. Exp. Cell. Res. 1972,71, 345.  

4 Mazur, P. Am. J. Physiol. 1984, 247, C125.  

5 Mazur, P. J. Gen. Physiol. 1963, 47, 347.  

6 Mansoori, G. A. Cryobiology 1975, 12, 34.  

7 Fahy, G. M. Biophys. J. 1980, 32, 837.

8 Fahy, G. M. Cryobiology 1981, 18, 473.  

9 Karlsson, J. O. M. J. Appl. Phys. 1984, 75, 4442.

10 Zhao, G.; Liu, Z.-F.; Yang, R.; Cheng, S.-X. Acta Chim. Sinica 2007, 65, 295 (in Chinese). (赵刚, 刘志峰, 杨锐, 程曙霞, 化学学报, 2007, 65, 295.)

11 Saenz, J.; Toner, M.; Risco, R. J. Phys. Chem. B 2009, 113,4853.  

12 Li, W.-B.; Zhao, G. Acta Chim. Sinica 2010, 68, 617 (in Chinese). (李卫兵, 赵刚, 化学学报, 2010, 68, 617.)

13 Rall, W. F.; Mazur, P.; McGrath, J. J. Biophys. J. 1983, 41,1.

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