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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 08: 1008 - 1012

DOI: https://doi.org/10.6023/A1108021

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Immobilization of Acetylcholinesterase on SnSe2 Hollow Spheres for Sensitive Detection of Phoxim

  • Zhang Can ,
  • Zhang Jinxing ,
  • Wang Kun ,
  • Dai Zhihui
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  • Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097

Received date: 2011-08-02

  Revised date: 2011-11-25

  Online published: 2011-12-14

Supported by

Project supported by the National Natural Science Foundation of China (No. 21175069), the Program for New Century Excellent Talents in University (No. NCET-09-0159) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Abstract

A simple method for immobilization of acetylcholinesterase (AChE) on SnSe2 hollow spheres for detection of phoxim was described. SnSe2 hollow spheres were prepared by hydrothermal methods and were characterized by TEM. The immobilized AChE retained its biological activity well and could catalyze the hydrolysis of acetylthiocholine to form thiocholine, which was then oxidized to produce detectable signal. Based on the inhibition toward the enzymatic activity of AChE by phoxim, under optimal conditions, the sensors could be used for the determination of phoxim ranging from 0.008 to 56 μg/mL with the detection limit of 0.004 μg/mL. The developed phoxim biosensors exhibited good stability and reproducibility. This work demonstrated that SnSe2 hollow sphere could be served as an ideal carrier for immobilization of AChE to fabricate corresponding biosensor.

Key words： acetylcholinesterase; SnSe2 hollow spheres; phoxim

Cite this article

Zhang Can , Zhang Jinxing , Wang Kun , Dai Zhihui . Immobilization of Acetylcholinesterase on SnSe2 Hollow Spheres for Sensitive Detection of Phoxim[J]. Acta Chimica Sinica, 2012 , 70(08) : 1008 -1012 . DOI: 10.6023/A1108021

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