Special Topic

Preparation and Application of Photoelectrochemical Guanine Sensor

  • Zhao Changzhi ,
  • Zhao Gaishuang ,
  • Zhang Zhaoxia ,
  • Liang Junyu
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  • College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology, Qingdao 266042, China

Received date: 2011-12-06

  Revised date: 2012-03-20

  Online published: 2012-03-20

Supported by

Project supported by the Natural Science Foundation of Shandong Province (No. 2009ZRB01393) and Electroanalytical Chemistry State Key Laboratory Fund (No. SKLEAC201106).

Abstract

We report herein a new guanine sensor for the determination of guanine based on a photoelectrochemical effect of poly(thionine) photoelectrode to hydrogen peroxide (H2O2). The photoelectrode was fabricated by electropolymerizing thionine on the surface of ITO electrode. And then xanthine oxidase was immobilized on the photoelectrode via the aid of chitosan enwrapping, forming an enzyme-modified photoelectrode. The photoelectrode was employed as an electron acceptor, and H2O2 from the catalytic reaction of enzyme was employed as an electron donor, the guanine can be detected due to photoelectrochemical effect between photoelectrode and H2O2. In the paper, photoelectrochemical effects of photoelectrode to H2O2 and guanine were studied. Effects of bias voltage, illumination intensity and electrolyte pH on the photocurrent were investigated. The linear response of guanine concentrations ranged from 1.00~200 μmol/L was obtained with a detection limit of 0.55 μmol/L. The guanine sensor was successfully applied to detect the disorganization of DNA mediated by heating acid solution and active ingredient of aciclovir. The relative standard deviation (RSD) was less than 5.37% and the recovery was in the range of 96.8%~106% for the determination of samples. The preparation of the sensor and the detection of guanine have the advantages of economy and simpleness due to without peroxidase and deoxygenated.

Cite this article

Zhao Changzhi , Zhao Gaishuang , Zhang Zhaoxia , Liang Junyu . Preparation and Application of Photoelectrochemical Guanine Sensor[J]. Acta Chimica Sinica, 2012 , 70(12) : 1401 -1406 . DOI: 10.6023/A1112061

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