SIOC 中文
ACS
Adv search

Acta Chimica Sinica >

2012 , Vol. 70 >Issue 16: 1748 - 1754

DOI: https://doi.org/10.6023/A12030074

Full Papers

Resonance Scattering Assay for Trace Human Chorionic Gonadotrophin Using Gold-platinum Nanoalloy Immunoprobe as Catalyst

  • Jiang Zhiliang ,
  • Yao Dongmei ,
  • Li Fang ,
  • Liang Aihui
Expand
  • Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Education Ministry;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry,Guangxi Normal University, Guilin 541004

Received date: 2012-03-30

  Online published: 2012-05-26

Supported by

Project supported by the National Natural Science Foundation of China (Nos. 21165005, 21075023) and the Fund of Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Ministry of Education of China (No. CMEMR2011-10).

Fold

Abstract

In the presence of sodium citrate as stabilizer, a gold-platinum nanoalloy (GP) in the mole ratio of 49∶1 was prepared by using NaBH4 as a reducing agent. The rabbit anti-human chorionic gonadotrophin antibody (RhCG) was labeled by the GP to obtain a stable immune GP probe (GP-RhCG) for the human chorionic gonadotrophin (hCG). In a pH 5.8 NaH2PO4-citric acid buffer solution and in the presence of KCl, the GP-RhCG probe was aggregated un-specifically to big particles that exhibited a resonance scattering (RS) peak at 590 nm. When the hCG was added, the GP-RhCG probe combined specifically with the hCG to form a dispersed GP-RhCG-hCG immunocomplex that resulted in the RS intensity decreasing at 590 nm. The decreased RS intensity (ΔI590 nm) is linear with hCG concentration in the range of 6.67—86.7 ng/mL, with a detection limit of 3.7 ng/mL hCG. The immunocomplex exhibited a strong catalytic effect on the slow Cu2O particle reaction of glucose-Cu(II), and the particles appeared a RS peak at 610 nm. When the hCG concentration increased in the immune reaction system, the immunocomplex concentration increased, and the catalytic product of Cu2O particles increased, which caused the enhancement of RS intensity at 610 nm. The increased intensity (ΔI610 nm) is linear with the hCG concentration in the range of 3.33—133 ng/mL, with a detection limit of 1.7 ng/mL hCG. In addition, the influence of foreign substances on the determination of hCG was examined. The results show that 150 times of L-aspartic acid, urea, BSA, glycine, Ca(II) and Zn(II), 50 times of L-lysine and L-cysteine, 30 times of IgG and IgA, 40 times of IgM did not interfered with the determination of 80 ng/mL hCG. This indicates that this method has good selectivity. The hCG content in the serum sample was analyzed by the GP RS method and GP-catalytic RS method, the results are agreement with those of the chemiluminescence experiments. Both RS methods are of some advantages including low-cost, simplicity, rapidity, high sensitivity and selectivity.

Key words： human chorionic gonadotrophin; gold-platinum nanoalloy; immunonanocatalysis; resonance scattering assay

Cite this article

Jiang Zhiliang , Yao Dongmei , Li Fang , Liang Aihui . Resonance Scattering Assay for Trace Human Chorionic Gonadotrophin Using Gold-platinum Nanoalloy Immunoprobe as Catalyst[J]. Acta Chimica Sinica, 2012 , 70(16) : 1748 -1754 . DOI: 10.6023/A12030074

References

[1] Sanvicens, N.; Marco, M. P. Trends Biotechnol. 2008, 26, 425.   

[2] Tongprasert, F.; Wanapirak, C.; Tongsong, T. Prenatal Diagn. 2011, 31, 430.   

[3] Cole, L. A.; Butler, S. A.; Khanlian, S. A.; Giddings, A.; Muller, C. Y.; Seckl, M. J.; Kohorn, E. I. Gynecol. Oncol. 2006, 102, 151.   

[4] Yang, H.-C.; Yuan, R.; Chai, Y.-Q.; Zhuo, Y. Colloids Surf., B 2011, 82, 463.   

[5] Chai, R.; Yuan, R.; Chai, Y.-Q.; Ou, C.-F.; Cao, S.-R.; Li, X.-L. Talanta 2008, 74, 1330.   

[6] Lim, T. K.; Matsunaga, T. Biosens. Bioelectron. 2001, 16, 1063.   

[7] Yang, G.-M.; Yang, X.-Y.; Yang, C.-Y.; Yang, Y.-H. Colloids Surf., A 2011, 389, 195.   

[8] Mao, L.; Yuan, R.; Chai, Y.-Q.; Zhuo, Y.; Yang, X. Sens. Actuators B 2010, 149, 226.

[9] Li, R.; Wu, D.; Li, H.; Xu, C.-X.; Wang, H.; Zhao, Y.-F.; Cai, Y.-Y.; Wei, Q.; Du, B. Anal. Biochem. 2011, 414, 196.

[10] Kim, H. S.; Pyun, J. C. Procedia Chem. 2009, 1, 1043.   

[11] Luo, L.; Zhang, Z.-J.; Hou, L.-Y.; Wang, J.-L.; Tian, W. Talanta 2007, 72, 1293.   

[12] Yan, X.; Huang, Z.-B.; He, M.; Liao, X.-M.; Zhang, C.-Q.; Yin, G.-F.; Gu, J.-W. Colloids Surf., B 2012, 89, 86.   

[13] Nakamura, N.; Lim, T. K.; Jeong, J. M.; Matsunaga, T. Anal. Chim. Acta 2001, 439, 125.

[14] Hudelist, G.; Wuelfing, P.; Czerwenka, K.; Knöfler, M.; Haider, S.; Fink-Retter, A.; Gschwantler-Kaulich, D.; Pfeiler, G.; Kubista, E.; Singer, C. F. J. Cancer Res. Clin. Oncol. 2009, 135, 191.

[15] Jiang, Z.-L.; Zou, M.-J.; Liang, A.-H. Clin. Chim. Acta 2008, 387, 24.

[16] Liang, A.-H.; Zou, M.-J.; Jiang, Z.-L. Talanta 2008, 75, 1214.   

[17] Nakamura, K.; Satomura, S.; Matsuura, S. Anal. Chem. 1993, 65, 613.

[18] Lv, M.-X.; Zhang, J.; Shen, Q.-Y.; Chen, K.-M.; Feng, G.-Y. Chin. J. Biomed. Eng. 1992, 11, 174. (吕鸣详, 张杰, 申群英, 陈昆明, 冯光易, 中国生物医学工程学报, 1992, 11, 174.)

[19] Robinson, G. A.; Hill, H. A.; Philo, R. D.; Gear, J. M.; Rattle, S. J.; Forrest, G. C. Clin. Chem. 1985, 31, 1449.

[20] Guo, X.-Y.; Guan, Y.-P.; Yang, B.; Wang, Y.-N.; Lan, H.-L.; Yang, Z.-H.; Lu, Z.-H. Int. J. Mol. Sci. 2006, 7, 274.

[21] Vaitukatitis, J. L.; Braunstein, G. D.; Ross, G. T. Am. J. Obstet. Gynecol. 1972, 113, 751.

[22] Tung, N. H.; Chikae, M.; Ukita, Y.; Viet, P. H.; Takamura, Y. Anal. Chem. 2012, 84, 1210.

[23] Liang, A.-H.; Huang, Y.-J.; Jiang, Z.-L. Clin. Chim. Acta 2007, 383, 73.

[24] Lin, S.-M.; Fan, Y.-Y.; Jiang, Z.-L.; Wen, G.-Q.; Liu, Q.-Y.; Liang, A.-H. Acta Chim. Sinica 2010, 68, 339. (凌绍明, 范燕燕, 蒋治良, 温桂清, 刘庆业, 梁爱惠, 化学学报, 2010, 68, 339.)

[25] Jiang, Z.-L.; Sun, S.-J.; Liang, A.-H.; Huang, W.-X.; Qin, A.-M. Clin. Chem. 2006, 52, 1389.

[26] Li, J.-F.; Jiang, Z.-L.; Deng, A.-P. Chin. J. Chem. 2008, 26, 2297.

[27] Jiang, Z.-L.; Fan, Y.-Y.; Liang, A.-H.; Wen, G.-Q.; Liu, Q.-Y.; Li, T.-S. Plasmonics 2010, 5, 375.   

[28] Jiang, C.-N.; Liang, A.-H.; Jiang, Z.-L. Acta Chim. Sinica 2011, 69, 714. (蒋彩娜, 梁爱惠, 蒋治良, 化学学报, 2011, 69, 714.)

[29] Zhang, Y.; Liang, A.-H.; Zhou, L.-P.; Qin, H.-M.; Ouyang, H.-X.; Wang, P.-F.; Jiang, Z.-L. Acta Chim. Sinica 2011, 69, 2153. (张轶, 梁爱惠, 周莲平, 覃惠敏, 欧阳辉祥, 王鹏飞, 蒋治良, 化学学报, 2011, 69, 2153.)

[30] Jiang, Z.-L.; Huang, Y.-J.; Liang, A.-H.; Pan, H.-C.; Liu, Q.-Y. Biosens. Bioelectron. 2009, 24, 1674. Jiang, Z.-L.; Liao, X.-J.; Deng, A.-P.; Liang, A.-H.; Li, J.-S.; Pan, H.-C.; Li, J.-F.; Wang, S.-M.; Huang, Y.-J. Anal. Chem. 2008, 80, 8681.

Options
Outlines

/

〈 〉