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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 12: 1379 - 1384

DOI: https://doi.org/10.6023/A1112156

Special Topic

A Comparison Study on the Interaction of Sunset Yellow and β-Carotene with Bovine Serum Albumin

  • Zhang Fangyuan ,
  • Ni Yongnian
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  • a State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047;
    b Department of Chemistry, Nanchang University, Nanchang 330031

Received date: 2011-12-15

  Revised date: 2012-03-19

  Online published: 2012-04-06

Supported by

Project was supported by the National Natural Science Foundation of China (No. 21065007) and the State Key Laboratory of Food Science and Technology of Nanchang University (Nos. SKLFMB-200807, SKLF-TS-200919).

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Abstract

Interaction of Sunset yellow (SY) and β-carotene (BC) with bovine serum albumin (BSA) was investigated under simulated physiological conditions by spectroscopic approaches (fluorescence, UV-Vis, FT-IR and CD). Both SY and BC quenched the intrinsic fluorescence of BSA through static quenching mechanism. The thermodynamic parameters (DH, DS, and DG) obtained from the fluorescence data measured at three different temperatures indicated that the binding of SY to BSA involved electrostatic force, and that of BC to BSA mainly by hydrogen bonding and van der Waals forces. The binding sites number n and binding constants Ka were also obtained. The result of FT-IR spectra and CD showed that the binding of SY to BSA induced conformational changes in BSA.

Key words： β-carotene; Sunset yellow; bovine serum albumin; molecular spectrometry; CD

Cite this article

Zhang Fangyuan , Ni Yongnian . A Comparison Study on the Interaction of Sunset Yellow and β-Carotene with Bovine Serum Albumin[J]. Acta Chimica Sinica, 2012 , 70(12) : 1379 -1384 . DOI: 10.6023/A1112156

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