Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging
Received date: 2012-07-24
Online published: 2012-09-11
Supported by
Project supported by the National Natural Science Foundation of China (Nos. 21033010, 21021091) and the Major Research Plan of China (Nos. 2011CB932302, 2011CB808400).
A new hydrophobic oligofluorene (OF) is synthesized via Suzuki cross-coupling reaction. The absorption and fluorescence spectra are measured in chloroform with high molar absorption coefficient (1.08?105 mol-1稬穋m-1) and fluorescence quantum yield (0.96). OF is dispersed into water to form nanoparticles with average size of 230 nm from dynamic light scattering (DLS) measurement. The nanoparticles in water still possess high molar absorption coefficient and fluorescence quantum yield. The quantitative cytotoxicity of OF nanoparticles to human lung cancer (A549) cells is measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, showing that OF nanoparticles exhibit low cellular toxicity. OF nanoparticles can be utilized for imaging of live A549 cells. Confocal laser scanning microscopy demonstrates that OF nanoparticles are exclusively located in the cytoplasm, especially around the perinuclear region. OF nanoparticles exhibit good overlap staining with lysosome-specific dye (Lyso Tracker Red), which demonstrates that OF nanoparticles could be used for lysosome-specific imaging.
Key words： conjugated oligomer; nanoparticles; cellular imaging; lysosome
Wen Quanshan , Tang Hongwei , Yang Gaomai , Liu Libing , Lv Fengting , Yang Qiong , Wang Shu . Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging[J]. Acta Chimica Sinica, 2012 , 70(20) : 2137 -2143 . DOI: 10.6023/A12070461
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