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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 21: 2208 - 2212

DOI: https://doi.org/10.6023/A12090629

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Rapid Site-Specific Protein Labeling Based on Diels-Alder Bioorthogonal Reactions

  • Wang Yue ,
  • Ye Xinshan
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  • State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191

Received date: 2012-09-05

  Online published: 2012-09-28

Supported by

Project supported by the National Basic Research Program of China (973 Program) (No. 2012CB822100) and the National Major Scientific and Technological Special Project for “Significant New Drugs Development” (No. 2012ZX09502001-001).

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Abstract

In recent years, many achievements have been made in site-specific protein modifications. This review highlights the latest advances on protein labeling, which utilizes the inverse electron-demand Diels-Alder bioorthogonal reactions of highly strained alkenes or alkynes and tetrazines, by genetically encoding unnatural amino acids containing one of these functionalities into the specific sites of proteins, leading to the rapid fluorescence labeling of cellular proteins.

Key words： protein labeling; fluorescence probe; bioorthogonal reactions; tetrazine; genetic encoding; unnatural amino acid

Cite this article

Wang Yue , Ye Xinshan . Rapid Site-Specific Protein Labeling Based on Diels-Alder Bioorthogonal Reactions[J]. Acta Chimica Sinica, 2012 , 70(21) : 2208 -2212 . DOI: 10.6023/A12090629

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