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Acta Chimica Sinica >

2013 , Vol. 71 >Issue 04: 602 - 612

DOI: https://doi.org/10.6023/A12100836

Article

Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase

  • Zhang Jianbo ,
  • Yang Yuxiang ,
  • Tian Meijuan ,
  • Deng Changjian ,
  • Cao Lei ,
  • Yuan Hongming
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  • a Department of Environmental Sciences, Peking University, Beijing 100871, China;
    b School of Chemistry and Molecular Engineering, East China University of Science & Technology,Shanghai 200237, China;
    c State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry,Jilin University, Changchun 130012, China

Received date: 2012-11-08

  Online published: 2013-02-01

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Abstract

Mesoporous silica with worm-like pore channels was synthesized using mixed P123 (polyethyleneoxide-polypropylene oxide-polyethylene oxide triblock copolymer EO20PO70EO20) and ionic cosurfactant {CTAB, SDS or N-myristoyl-D-alanine (C14-D-A)} as a structure-directing agent, and the pore-expanding effect of different amount of PEG-400 on the worm-like pore channels was studied. The synthesized products were characterized using X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and nitrogen sorption analysis. Moreover, the worm-like pore-expanded mesoporous silica was extracted with solvent, and the obtained quaternary ammonium functionalized mesoporous silica with different pore size was applied to immobilize laccase respectively. The stability of immobilized laccase, the optimum pH value acquired and reusability for the degradation of 2,4-DCP were also studied. Through comparative studies, it is found that, matching of sizes between the enzyme molecule and the pore diameter of the mesoporous silica, is essential for specific activity of immobilized laccase and degradation efficiency of 2,4-DCP by immobilized laccase.

Key words： worm-like mesoporous silica; pore-expanding; 2,4-DCP; degradation efficiency; reusability

Cite this article

Zhang Jianbo , Yang Yuxiang , Tian Meijuan , Deng Changjian , Cao Lei , Yuan Hongming . Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase[J]. Acta Chimica Sinica, 2013 , 71(04) : 602 -612 . DOI: 10.6023/A12100836

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