Construction and Performance of a New Bioanode for Biofuel Cells
Received date: 2013-03-15
Online published: 2013-06-13
Supported by
Project supported by the National Natural Science Foundation of China (No. 21275041), Hunan Provincial Natural Science Foundation of China (No. 12JJ2010), the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20110161110009), and Program for Changjiang Scholars and Innovative Research Team in University (No. IRT1238).
Acid-treated carbon nanotubes (CNTs) were functionalized by β-cyclodextrin (CD) through the van der Waals force between them. It is well-known that CD is toroidal in shape with a hydrophobic inner cavity and a hydrophilic exterior. Based on the recognition of CD to ferrocene (Fc), a water-soluble complex, CNTs-β-CD-Fc, was prepared. Glucose oxidase (GOD) was mixed with CNTs-β-CD-Fc and crosslinked with glutaraldehyde to form enzyme polymer. Then the GOD/CNTs-β-CD-Fc composite was coated on the glassy carbon (GC) electrode and the GOD/CNTs-β-CD-Fc/GC bioanode was obtained. The prepared CNTs-β-CD and CNTs-β-CD-Fc were characterized by thermal gravimeritric analysis, fourier transform infrared spectroscopy and transmission electron microscopy. The electrocatalytic properties of the GOD/CNTs-β-CD-Fc/GC bioanode towards glucose oxidation were investigated by cyclic voltammetry. The results showed that under the same experimental conditions, the GOD/CNTs-β-CD/GC electrode (without Fc) had almost no catalytic currents for glucose oxidation and the GOD/CNTs-β-CD-Fc/GC bioanode had higher electrocatalytic activity towards glucose oxidation than the GOD/CNTs-Fc/GC electrode. The long-term cycle stability of the developed GOD/CNTs-β-CD-Fc/GC bioanode was also evaluated by cyclic voltammetry and the results showed that the GOD/CNTs-β-CD-Fc/GC bioanode had much better stability than the GOD/CNTs-Fc/GC electrode. Taking the commercial E-TEK Pt/C modified GC electrode as the cathode, the maximum power density of the glucose/O2 biofuel cell (EBFC) based on the GOD/CNTs-β-CD-Fc/GC anode was 33 μW·cm-2 (at 0.18 V), almost 3 times higher than that of the EBFC based on the GOD/CNTs-Fc/GC anode (11.7 μW·cm-2 at 0.16 V). The stability of the developed EBFC was also investigated by monitoring the change of the open circuit potential (OCP) of EBFC. After continuous operation for 9 h, the developed EBFC (the GOD/CNTs-β-CD-Fc/GC electrode as the bioanode) remained 92% of initial OCP, suggesting the good operating stability. The proposed strategy for bioanode preparation may provide a new way for the development of glucose/O2 EBFC with good perfomance and high stability.
Key words： carbon nanotubes; ferrocene; β-cyclodextrin; glucose oxidase; biofuel cells
Zou Qiong , Liu Juan , Zhu Gangbing , Zhang Xiaohua , Chen Jinhua . Construction and Performance of a New Bioanode for Biofuel Cells[J]. Acta Chimica Sinica, 2013 , 71(08) : 1154 -1160 . DOI: 10.6023/A13030285
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