CdTe quantum dots (QDs) and CdTe(QDs) sensitized-TiO₂ nanocomposites were synthesized by hydrothermal and self-assembly method, and the suspension of CdTe(QDs)/TiO₂ and TiO₂ were dubbed into the concentration of 1×10^-2 mol/L. When a small ring of nutrient broth on E. coli was added into 5 mL LB medium, then E. coli was dubbed approximately the concentration of 1×10⁶ cell/mL. The effect of CdTe(QDs)/TiO₂ nanocomposites on E. coli was evaluated by a LKB-2277 Bioactivity Monitor using the stopped-flow method at 37.0 ℃. By analyzing the thermogenic power-time curves, thermokinetic parameters such as growth rate constant (k), maximum heat power of growth phase (P_m), generation time (t_G) and inhibitory ratio (I) were determined. The results showed that both k and I have linear correlations with the concentration of nanocomposites in the scope of 0～4.0×10^-5 mol·L^-1. The value of k and P_m decrease while t_G and I increase with increasing concentrations of the CdTe(QDs)/TiO₂ nanocomposites added into the microbe suspension. Wherein, the slope of growth rate constant (k) on CdTe(QDs)/TiO₂ is less than the slope of growth rate constant (k) on TiO₂, can you initially determine CdTe(QDs)/TiO₂ nanocomposites has larger effect than TiO₂ on E. coli. To some extent, CdTe(QDs)/TiO₂ nanocomposites and TiO₂ have the metabolic activity on E. coli, the slope of inhibitory ratio (I) (0.84) on CdTe(QDs)/TiO₂ is greater than the slope of inhibitory ratio (I) (0.26) on TiO₂ at the same concentration. Compared with TiO₂, CdTe(QDs)/TiO₂ has higher antibacterial activity on E. coli. Combining the visual method of Hadama fluorescence microscopy. The CdTe(QDs)/TiO₂ nanocomposites shows a better antibacterial activity on E. coli than that of nano-TiO₂ because of the changes of surface structure. Our results also provide theoretical brace in order to investigate further on the impact of nanomaterials on the metabolic processes of microbial growth and the kinetics and mechanisms of cell damage.