Preparation and Adsorption Properties Study of Glucose Magnetic Molecularly Imprinted Polymers with Dual Functional Monomers
Received date: 2015-09-29
Online published: 2015-12-23
Supported by
Project supported by the Outstanding Academic Leaders of Harbin (No. 2014RFXXJ113).
In this paper, dual functional monomers (concanavalin A and aminobenzeneboronic acid) method is applied to molecular imprinting technique, three synthetic methods of functional monomer were designed and their ability to adsorb glucose was evaluated. Step-by-step method (A1) was found to be a good synthetic method for selective adsorption, which could adsorb glucose powerfully. A kind of magnetic molecularly imprinted polymers (MMIPs) was synthesized by step-by-step method, in which superparamagnetic Fe3O4 nanoparticles were firstly synthesized by hydrothermal synthetic method and uniform SiO2-coated Fe3O4 (Fe3O4@SiO2) nanoparticles were prepared via Sol-gel method. The following Fe3O4@SiO2 reacted with (3-aminopropyl)triethoxysilane to produce Fe3O4@SiO2@NH2. The aminated nanoparticles combine with concanavalin A to form Fe3O4@SiO2@NH2@ConA in the presence of disuccinimidyl suberate. Subsequently, the single-functionalized nanoparticles binded with aminobenzeneboronic acid to form Fe3O4@SiO2@NH2@APBA-ConA in the presence of disuccinimidyl suberate. Eventually, the MMIPs for glucose selective recognition, with core-shell structure, were synthesized by surface molecular imprinting method, using glucose (Glu) as template molecule, concanavalin A (ConA) and aminobenzeneboronic acid (APBA) as dual functional monomers, N,N'-methylenebisacrylamide as the crosslinking agent, ammonium peroxydisulfate as the initiator, superparamagnetic nanoparticles as magnetic carrier. The MMIPs were characterized by X-ray diffraction (XRD), energy dispersive spectrometer (EDS), scanning electron microscopy (SEM), Fourier transform infrared spectra (FT-IR), vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA). The SEM showed the nanoparticles were highly dispersive and uniform. The EDS, FT-IR and TGA revealed the success of step-by-step method. The VSM demonstrated the saturation magnetization values of Fe3O4 and MMIPs were 96.661 emu/g and 45.064 emu/g, respectively. The study on kinetics of MMIPs showed that the adsorption reaction could be considered as a second order reaction. The thermodynamics research revealed that the adsorption of MMIPs was fitted to Langmuir isotherms. The study on selective adsorption displayed that MMIPs had maximum imprinting factor of 2.93 and best selectivity. The class specific study of MMIPs showed that it could be applied to extraction and separation of glucosides.
Zhi Kangkang , Dong Aijun , Yang Xin , Zhao Qianyu , Zhao Haitian , Zhang Hua , Wang Jing , Xu Pengfei . Preparation and Adsorption Properties Study of Glucose Magnetic Molecularly Imprinted Polymers with Dual Functional Monomers[J]. Acta Chimica Sinica, 2016 , 74(2) : 199 -207 . DOI: 10.6023/A15090636
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