Chiral Gold Nanoclusters: A New Near-Infrared Fluorescent Probe
Received date: 2016-01-19
Online published: 2016-04-08
Supported by
Project supported by China National Funds for Distinguished Young Scientists (Grant No. 51325302) and the National Natural Science Foundation of China (Grant Nos. 51533007, 51521001).
Near-infrared (NIR) fluorescence facilitates noninvasive bio-imaging because it involves less interference from blood and tissue auto-fluorescence and high transparency. Nowadays, the research of new NIR fluorescent probes with favorable biocompatibility, high quantum yield, high stability and long-wavelength emission band has become the focus of bio-nanotechnology. Herein, we introduced NIBC enantiomers onto the surface of gold nanoclusters and synthesized chiral gold nanoclusters anchored with N-isobutyryl-L-cysteine (L-NIBC-AuNCs) and N-isobutyryl-D-cysteine (D-NIBC-AuNCs), respectively. Transmission electron microscopy (TEM images) of the L-NIBC-AuNCs and D-NIBC-AuNCs reveal that the particle sizes of both two AuNCs are around 1.9±0.7 nm. The UV-Vis absorption spectra of L-NIBC-AuNCs and D-NIBC-AuNCs are basically identical and both two AuNCs have characteristic absorption peaks at 580 nm and 680 nm. Compared with the FT-IR spectra of NIBC, the vanishing of the S—H stretching vibration at the 2500~2600 cm-1 in the FT-IR spectra of L-NIBC-AuNCs and D-NIBC-AuNCs indicate that L-NIBC and D-NIBC have successfully anchored on to the surface of Au core by Au—S bond. The circular dichroism (CD) spectra of L-NIBC-AuNCs and D-NIBC-AuNCs show nearly a mirror image relationship at 230~360 nm, which means the chirality signal transmitted from molecular level to nanoscale level. Most important of all, both two water-soluble nanoclusters have fluorescence emission bands between 900~1000 nm which belong to the near infrared bands. And the fluorescence quantum yields of L-NIBC-AuNCs and D-NIBC-AuNCs are 6.9% and 8.2%, respectively. Cell toxicity experiments show that both two kinds of gold nanoclusters have no cytotoxicity even at the high concentration of 100 mg/L. Moreover, these gold nanoclusters also have unique chiroptical activity and potential chiral recognition ability. Based on the experiment mentioned above, these kinds of chiral gold nanoclusters can be used as a new kind of near-infrared fluorescent probe, which may have promising application in the near-infrared fluorescent imaging. These findings provide an interesting insight in the near-infrared fluorescence (NIRF) imaging techniques.
Key words： gold nanoclusters; near-infrared; fluorescent probe; chirality
Gao Guanbin , Gong Dejun , Zhang Mingxi , Sun Taolei . Chiral Gold Nanoclusters: A New Near-Infrared Fluorescent Probe[J]. Acta Chimica Sinica, 2016 , 74(4) : 363 -368 . DOI: 10.6023/A16010038
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