Au Nanocages Probes for Mitochondrial Imaging and Photothermal Damage Cells★
Received date: 2023-05-12
Online published: 2023-07-21
Supported by
National Natural Science Foundation of China(22276102); Young Taishan Scholar Program of Shandong Province of China(tsqn202211212); Shandong Provincial Natural Science Foundation(ZR2023JQ004); Shandong Provincial Natural Science Foundation(ZR2022MB024); University Institute Innovation Team of Jinan(202228027); Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province(2020KJC003); Science, Education and Industry Integration Pilot Project Plan of Qilu University of Technology (Shandong Academy of Sciences)(2023PYI002)
Mitochondria are the key regulatory organelles of many cell behaviors, and the reduction of mitochondrial membrane potential is considered to be one of the earliest events of cell apoptosis. Therefore, mitochondrial imaging and the detection and analysis of mitochondrial membrane potential are of great scientific significance for the detection and treatment of diseases. In this work, AuNCs/PLEL/JC/KLA, a mitochondrial targeted fluorescent nanoprobe, was developed using Au nanocages (AuNCs) mediated photothermal damage combined with temperature-sensitive drug release. At the same time, the temperature-sensitive hydrogel poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide) (PDLLA-PEG-PDLLA, PLEL) is used as the outer structure to control the release. A mitochondrial targeting peptide (KLAKLAKKLAKLAK, KLA) was introduced as the "pointer" of the nanoprobe to specifically target the mitochondria. The colocalization experiment showed that the nanoprobe was highly colocalized with mitochondria, indicating that the nanoprobe was selectively enriched in mitochondria. It is worth noting that the nanoprobe has excellent photothermal properties, and its photothermal conversion efficiency can be as high as 39.11%. Therefore, under the irradiation of near infrared light, the probe can absorb light energy into heat. Subsequently, the results of Cell Counting Kit 8 (CCK-8) confirmed that the nanoprobes could achieve local photothermal damage at mitochondrial sites, triggering high temperature mediated mitochondrial dysfunction and inducing apoptosis of cancer cells. Meanwhile, rheological analysis and fluorescence curve showed that high temperature promotes the gel-sol transformation of PLEL thermosensitive hydrogel and realizes the release of fluorescent dye (JC-10). The confocal images of the cells showed that the released JC-10 fluorescent dye can display red and green fluorescence signals based on mitochondrial activity. In conclusion, the fluorescence nanoprobe can not only achieve mitochondrial targeted fluorescence imaging and damage cells, but also monitor the changes of mitochondrial membrane potential.
Li Sun , Yajing Wang , Tao Li , Yingshu Guo , Shusheng Zhang . Au Nanocages Probes for Mitochondrial Imaging and Photothermal Damage Cells★[J]. Acta Chimica Sinica, 2023 , 81(10) : 1301 -1310 . DOI: 10.6023/A23050222
| [1] | Al Kelabi, D.; Dey, A.; Alimi, L. O.; Piwonski, H.; Habuchi, S.; Khashab, N. M. Chem. Sci. 2022, 13, 7341. |
| [2] | Anishiya Chella Daisy, E. R.; Rajendran, N. K.; Jeyaraj, M.; Ramu, A.; Rajan, M. J. Liposome Res. 2021, 31, 203. |
| [3] | Asadian-Birjand, M.; Bergueiro, J.; Wedepohl, S.; Calderon, M. Macromol. Biosci. 2016, 16, 1432. |
| [4] | Cao, M. X.; Dai, X. G.; Chen, B. B.; Zhao, N. N.; Xu, F. J. Acta Chim. Sinica 2020, 78, 1054 (in Chinese). |
| [4] | (曹萌轩, 代晓光, 陈贝贝, 赵娜娜, 徐福建, 化学学报, 2020, 78, 1054.) |
| [5] | Bae, Y.; Joo, C.; Kim, G. Y.; Ko, K. S.; Huh, K. M.; Han, J.; Choi, J. S. Macromol. Res. 2019, 27, 1071. |
| [6] | Bouchaala, R.; Anton, N.; Anton, H.; Vandamme, T.; Vermot, J.; Smail, D.; Mely, Y.; Klymchenko, A. S. Colloid Surface B 2017, 156, 414. |
| [7] | Wu, D.; Wang, Y. J.; Wang, Q. Q.; Li, T.; Guo, Y. S. Chin. J. Analysis Laboratory 2023, DOI: 10.13595/j.cnki.issn1000-0720.2023.032703 (in Chinese). |
| [7] | (吴谛, 王亚静, 王倩倩, 李涛, 郭英姝, 分析试验室, 2023, DOI: 10.13595/j.cnki.issn1000-0720.2023.032703.) |
| [8] | Cai, Y.; Zhu, H. S.; Zhou, W. C.; Qiu, Z. Y.; Chen, C. C.; Qileng, A.; Li, K. S.; Liu, Y. J. Anal. Chem. 2021, 93, 7275. |
| [9] | Chen, H. C.; Wang, Y. R.; Yao, Y. Y.; Qiao, S. L.; Wang, H.; Tan, N. H. Theranostics 2017, 7, 3781. |
| [10] | Chen, M. M.; Li, R. Y.; Liu, Y.; Song, X. R.; Tian, J.; Fu, Y. L.; Yang, Y. J.; Liu, C.; Zhang, Q. Q. Chem. Eng. J. 2021, 406, 126745. |
| [11] | Chen, S.; Lei, Q.; Qiu, W. X.; Liu, L. H.; Zheng, D. W.; Fan, J. X.; Rong, L.; Sun, Y. X.; Zhang, X. Z. Biomaterials 2017, 117, 92. |
| [12] | Chen, S. B.; Zhong, H.; Gu, B.; Wang, Y. Z.; Li, X. M.; Cheng, Z. P.; Zhang, L. L.; Yao, C. Mat. Sci. Eng. C-Mater. 2012, 32, 2199. |
| [13] | Chen, W.; Shi, K.; Liu, J.; Yang, P. P.; Han, R. X.; Pan, M.; Yuan, L. P.; Fang, C.; Yu, Y. Y.; Qian, Z. Y. Bioact. Mater. 2023, 23, 1. |
| [14] | Zhou, H. M.; Tang, Y. H.; Lu, H. X.; Zhang, Q.; Lin, W. Y. Chin. J. Org. Chem. 2022, 42, 1687 (in Chinese). |
| [14] | (周红梅, 唐永和, 卢辉旭, 张倩, 林伟英, 有机化学, 2022, 42, 1687.) |
| [15] | Chen, X. M.; Chen, D. R.; Liu, H. M.; Yang, L.; Zhang, Y. T.; Bu, L. L.; Sun, Z. J.; Cai, L. L. J. Control. Release 2022, 351, 381. |
| [16] | Chi, J. N.; Ma, Q. M.; Shen, Z. J.; Ma, C. Y.; Zhu, W.; Han, S. C.; Liang, Y.; Cao, J.; Sun, Y. Nanoscale 2020, 12, 11008. |
| [17] | Chuang, C. H.; Chen, W. Y.; Tseng, W. B.; Lin, A. Y.; Lu, C. Y.; Tseng, W. L. ACS Sustainable Chem. Eng. 2022, 10, 2461. |
| [18] | Guo, Y. S.; Cao, X. P.; Zhang, S. S. Chem. Commun. 2021, 57, 10504. |
| [19] | Guo, Y. S.; Zheng, X. F.; Gai, T. T.; Wei, Z. Y.; Zhang, S. S. Chem. Commun. 2021, 57, 5754. |
| [20] | Guo, Y. S.; Wang, Y. J.; Li, S.; Niu, L.; Wei, D.; Zhang, S. S. Chem. Commun. 2017, 53, 4826. |
| [21] | Liu, F.; Guo, Y. S.; Hu, Y. H.; Zhang, X. R.; Zheng, X. J. Anal. Bioanal. Chem. 2019, 411, 5845. |
| [22] | Guo, Y. S.; Zheng, X. F.; Cao, X. P.; Li, W. X.; Wu, D.; Zhang, S. S. Chin. J. Chem. 2020, 38, 1793. |
| [23] | Hu, X. J.; Gao, G. B.; Zhang, M. X. Acta Phys.-Chim. Sin. 2017, 33, 1324 (in Chinese). |
| [23] | (胡雪娇, 高冠斌, 张明曦, 物理化学学报, 2017, 33, 1324.) |
| [24] | Li, Y. R.; Wang, Z. G.; Tang, C. H. Acta Chim. Sinica 2022, 80, 291 (in Chinese). |
| [24] | (李嫣然, 王子贵, 汤朝晖, 化学学报, 2022, 80, 291.) |
| [25] | Zheng, B. X.; Bi, C. F.; Hou, W. B.; Li, W. L. Acta Pharm. Sin. 2022, 57, 681 (in Chinese). |
| [25] | (郑宝鑫, 毕常芬, 侯文彬, 李祎亮, 药学学报, 2022, 57, 681.) |
| [26] | Guo, X. L.; Yang, N. D.; Ji, W. H.; Zhang, H.; Dong, X.; Zhou, Z. Q.; Li, L.; Shen, H. M.; Yao, S. Q.; Huang, W. Adv. Mater. 2021, 33, 2007778. |
| [27] | Zhang, W.; Du, X. F.; Liu, B.; Li, C. R.; Long, J.; Zhao, M. X.; Yao, Z. Y.; Liang, X. J.; Lai, Y. X. ACS Nano 2022, 16, 1421. |
| [28] | Hu, X. W.; Zhang, J.; Yu, Z.; Xie, Y. C.; He, H. S.; Qi, J. P.; Dong, X. C.; Lu, Y.; Zhao, W. L.; Wu, W. Nanomedicine 2015, 11, 1939. |
| [29] | Huang, W. Q.; Wang, F.; Nie, X.; Zhang, Z.; Chen, G.; Xia, L.; Wang, L. H.; Ding, S. G.; Hao, Z. Y.; Zhang, W. J.; Hong, C. Y.; You, Y. Z. ACS Appl. Bio. Mater. 2020, 3, 1176. |
| [30] | Jeevarathinam, A. S.; Lemaster, J. E.; Chen, F.; Zhao, E.; Jokerst, J. V. Angew. Chem., Int. Ed. 2020, 59, 4678. |
| [31] | He, H. M.; Zhao, F.; Zhong, W. K.; Yang, Y. L.; Lin, Y. H.; Ding, Y. H.; Yang, J. J.; Lu, C. H.; Tu, X. K. Mater. Design 2022, 219, 110722. |
| [32] | Horton, K. L.; Stewart, K. M.; Fonseca, S. B.; Guo, Q.; Kelley, S. O. Chem. Biol. 2008, 15, 375. |
| [33] | Fan, X. H.; Wang, Y.; Yang, Y. Y.; Zhang, Y. H. Chem. J. Chin. Univ. 2022, 43, 168 (in Chinese). |
| [33] | (樊晓慧, 汪洋, 杨园园, 张玉红, 高等学校化学学报, 2022, 43, 168.) |
| [34] | Hu, W. Y.; Zhou, C. T.; Jing, Q. G.; Li, Y. C.; Yang, J.; Yang, C.; Wang, L. Y.; Hu, J. Y.; Li, H. J.; Wang, H. R.; Yuan, C.; Zhou, Y.; Ren, X. Y.; Tong, X. M.; Du, J.; Wang, Y. Cancer Cell Int. 2021, 21, 709. |
| [35] | Zhang, Z. P.; Xu, S. H.; Wang, Y.; Yu, Y. N.; Li, F. Z.; Zhu, H.; Shen, Y. Y.; Huang, S. T.; Guo, S. R. J. Colloid Inter. Sci. 2018, 509, 47. |
| [36] | Jia, Y. P.; Shi, K.; Yang, F.; Liao, J. F.; Han, R. X.; Yuan, L. P.; Hao, Y.; Pan, M.; Xiao, Y.; Qian, Z. Y.; Wei, X. W. Adv. Funct. Mater. 2020, 30, 2001059. |
| [37] | Qiu, M. L.; Chen, D. Y.; Shen, C. Y.; Shen, J.; Zhao, H. K.; He, Y. H. Int. J. Mol. Sci. 2016, 17, 2001442. |
| [38] | Jeong, C.; Uthaman, S.; Bagheri, B.; Kim, J.; Pillarisetti, S.; Park, I. K.; Kim, Y. C. J. Control. Release 2021, 329, 50. |
| [39] | Jia, H. Y.; Yang, L.; Fan, D. W.; Kuang, X.; Sun, X.; Wei, Q.; Ju, H. X. Sensor. Actuat. B-Chem. 2022, 367, 132034. |
| [40] | Jiang, L.; Zhou, S. S.; Zhang, X. K.; Li, C.; Ji, S. L.; Mao, H.; Jiang, X. Q. Nat. Commun. 2021, 12, 2390. |
| [41] | Jiao, Y.; Guo, Y. M.; Fan, Y. C.; Wang, R.; Li, X.; Wu, H.; Meng, Z. C.; Yang, X.; Cui, Y. P.; Liu, H.; Pan, L. P.; Maimaitijuma, T.; Zhang, J. Z.; Wang, Y. H.; Cao, Y. P.; Zhang, T. Bio. Med. Res. Int. 2020, 2020, 2846297. |
| [42] | Kadkhoda, J.; Tarighatnia, A.; Nader, N. D.; Aghanejad, A. Life Sci. 2022, 307, 120898. |
| [43] | Ke, L. B.; Zhang, C.; Liao, X. X.; Qiu, K. Q.; Rees, T. W.; Chen, Y.; Zhao, Z. Z.; Ji, L. N.; Chao, H. Chem. Commun. 2019, 55, 10273. |
| [44] | Kolygina, D. V.; Siek, M.; Borkowska, M.; Ahumada, G.; Barski, P.; Witt, D.; Jee, A. Y.; Miao, H.; Ahumada, J. C.; Granick, S.; Kandere-Grzybowska, K.; Grzybowski, B. A. ACS Nano 2021, 15, 11470. |
| [45] | Skrabalak, S. E.; Chen, J. Y.; Au, L. L.; Lu, X. M.; Li, X. D.; Xia, Y. N. Adv. Mater. 2007, 19, 3177. |
| [46] | Skrabalak, S. E.; Au, L. L.; Lu, X. M.; Li, X. D.; Xia, Y. N. Nanomedicine 2007, 2, 657. |
| [47] | Zhou, X. H.; He, X. L.; Shi, K.; Yuan, L. P.; Yang, Y.; Liu, Q. Y.; Ming, Y.; Yi, C.; Qian, Z. Y. Adv. Sci. 2020, 7, 2001442. |
| [48] | Luo, Y.; Yang, L.; Feng, P. P.; Qiu, H. F.; Wu, X. J.; Lu, S. W.; Zhou, M.; Xu, L.; Zhu, Y. B. Front. Bioeng. Biotech. 2020, 8, 581621. |
| [49] | Zhang, Z. J.; Zhang, X. L.; Wang, C. G.; Teng, W. S. Y.; Xing, H. Y.; Wang, F. Q.; Yinwang, E.; Sun, H. X.; Wu, Y.; Yu, C. C.; Chai, X. P.; Qian, Z. Q.; Yu, X. H.; Ye, Z. M.; Wang, X. Y. Chem. Eng. J. 2022, 433, 134465. |
| [50] | Xu, Z.; Liu, Y. J.; Ma, R.; Chen, J.; Qiu, J. M.; Du, S.; Li, C. C.; Wu, Z. H.; Yang, X. F.; Chen, Z. B.; Chen, T. K. ACS Appl. Mater. Interfaces 2022, 14, 14059. |
| [51] | Zheng, Z. Q.; Bian, S. Q.; Li, Z. Q.; Zhang, Z. Y.; Liu, Y.; Zhai, X. Y.; Pan, H. B.; Zhao, X. L. Carbohydr. Polym. 2020, 249, 116826. |
| [52] | Wan, J. S.; Geng, S. N.; Zhao, H.; Peng, X. L.; Xu, J. B.; Wei, M. H.; Mao, J. X.; Zhou, Y.; Zhu, Q.; Zhao, Y. B.; Yang, X. L. Nanoscale 2018, 10, 20020. |
| [53] | Wang, X. H.; Wang, K. X.; Liu, J. P.; Hong, X. Chem. J. Chin. Univ. 2019, 40, 1586 (in Chinese). |
| [53] | (王晓慧, 王可心, 刘俊平, 洪霞, 高等学校化学学报, 2019, 40, 1586.) |
| [54] | Shao, J. D.; Ruan, C. S.; Xie, H. H.; Li, Z. B.; Wang, H. Y.; Chu, P. K.; Yu, X. F. Adv. Sci. 2018, 5, 1700848. |
| [55] | Tang, Q.; Lim, T.; Shen, L. Y.; Zheng, G.; Wei, X. J.; Zhang, C. Q.; Zhu, Z. Z. Biomaterials 2021, 268, 120605. |
| [56] | Li, W. X.; Wang, Y. J.; Sun, L.; Li, T.; Guo, Y. S. Chin. J. Analysis Laboratory 2023, DOI: 10.13595/j.cnki.issn1000-0720.2023.041602 (in Chinese). |
| [56] | (李文鑫, 王亚静, 孙丽, 李涛, 郭英姝, 分析试验室, 2023, DOI: 10.13595/j.cnki.issn1000-0720.2023.041602.) |
/
| 〈 |
|
〉 |
