Chin. J. Org. Chem. ›› 2018, Vol. 38 ›› Issue (10): 2706-2712.DOI: 10.6023/cjoc201803050 Previous Articles     Next Articles

Articles

表面修饰适配体S6的聚乳酸-羟基乙酸/聚乙二醇共聚物纳米粒制备及其运载小干扰RNA的应用研究

刘洋a, 谢栓栓a, 曾洁a, 谈敏a, 宋小莲a, 朱君b, 王昌惠a   

  1. a 同济大学附属上海市第十人民医院 上海 200072;
    b 纳米技术及应用国家工程研究中心 上海 200241
  • 收稿日期:2018-03-29 修回日期:2018-06-19 发布日期:2018-06-22
  • 通讯作者: 王昌惠,E-mail:wangch@tongji.edu.cn;朱君,E-mail:yzjzhu@163.com E-mail:wangch@tongji.edu.cn;yzjzhu@163.com
  • 基金资助:

    国家自然科学基金(No.81472180)资助项目.

Preparation of Poly (lactic-co-glycolic acid)/Polyethylene Glycol Nanoparticles with Surface-Modified Aptamer S6 and Its Application on Carrier siRNA

Liu Yanga, Xie Shuanshuana, Zeng Jiea, Tan Mina, Song Xiaoliana, Zhu Junb, Wang Changhuia   

  1. a Department of Respiratory Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072;
    b National Engineering Research Center for Nanotechnology, Shanghai 200241
  • Received:2018-03-29 Revised:2018-06-19 Published:2018-06-22
  • Contact: 10.6023/cjoc201803050 E-mail:wangch@tongji.edu.cn;yzjzhu@163.com
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 81472180).

Lung cancer threats human health. The poly (lactic-co-glycolic acid)/polyethylene glycol (PLGA-PEG) was synthesized by the coupling method and aptamer S6 was synthesized by the nucleic acid solid-phase phosphorimide method. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide salt was obtained. Acid-mediated (EDC) and N-hydroxysuccinimide (NHS) mediators have resulted in a biotargeting nanocarrier material PLGA-PEG-S6-CY3. PLGA-PEG-S6-CY3/CAP1-siRNA nanoparticles were constructed by carrying the small interfering RNA (CAP1-siRNA) targeting the adenylate cyclase-associated protein 1 through the carrier material, and the sustained release of the CAP1-siRNA was achieved to achieve targeting inhibition of metastasis of non-small cell lung cancer A549 cells. The molecular structures of S6 and PLGA-PEG were characterized by nuclear magnetic resonance spectroscopy and their targeting properties were verified. The binding and release of PLGA-PEG nanoparticles and CAP1-siRNA were detected. Furthermore, the effect of the complex on inhibiting the invasiveness of tumor cells was further investigated. The results showed that the PLGA-PEG molecule prepared by the coupling method was used as the carrier, and the aptamer S6 was synthesized and used to modify PLGA-PEG. The obtained PLGA-PEG nanocomposite has the ability of targeting and carrying siRNA, and can be used in vitro. It can also down-regulate the expression of CAP1 in lung cancer A549 cells and reduce its invasiveness.

Key words: aptamer, PLGA-PEG molecule, siRNA, anti-tumor invasion References