Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (8): 2374-2386.DOI: 10.6023/cjoc202001021 Previous Articles     Next Articles


张燕a, 王芸芸a, 赵雨珣a, 张成龙a, 谷文a, 王忠龙a, 朱永强b, 王石发a   

  1. a 南京林业大学化学工程学院 南京林业大学林业资源高效加工利用协同创新中心 南京 210037;
    b 江苏正大丰海制药有限公司 南京 210033
  • 收稿日期:2020-01-14 修回日期:2020-05-22 发布日期:2020-06-10
  • 通讯作者: 王石发, 朱永强;
  • 基金资助:

Camphor-Based Thiosemicarbazone Analogues Induced G2 Cell Cycle Arrest and Apoptosis via Reactive Oxygen Species (ROS)-Mediated Mitochondrial Pathway in Human Breast Cancer Cells

Zhang Yana, Wang Yunyuna, Zhao Yuxuna, Zhang Chenglonga, Gu Wena, Wang Zhonglonga, Zhu Yongqiangb, Wang Shifaa   

  1. a Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037;
    b Jiangsu Chia Tai Fenghai Pharmaceutical Co. Ltd, Nanjing 210033
  • Received:2020-01-14 Revised:2020-05-22 Published:2020-06-10
  • Supported by:
    Project supported by the Doctorate Fellowship Foundation of Nanjing Forestry University, the Natural National Science Foundation of China (No. 31470592), the University Science Research Project of Jiangsu Province (No. 14KJ220001) and the Key Technology of Green Processing and Efficient Utilization on Oleoresin (No. 2016YFD0600804).

22 novel camphor-based thiosemicarbazone derivatives were synthesized using camphor-based thiosemicarbazone as material and their structures were determined by 1H NMR, 13C NMR and HRMS. The crystal structure of 2-(3-(pyridin-4-ylmethylene)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-ylidene)hydrazinecarbothioamide (3n) was determined by single crystal X-ray diffraction. The derivatives were screened in vitro for anticancer activities against human breast cancer cell line (MDA-MB-231), human lung adenocarcinoma cell line (A549), human multiple myeloma cell line (RPMI-8226) and toxicity against a normal human cell line (GES-1) by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS). It was found that majority of the tested analogs showed moderate to significant antitumor activity against selected cancer cell lines. Noticeably, 2-(3-(anthracen-9-ylmethylene)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-ylidene)-hydrazinecarbothioamide (3s) exhibited selective anti-tumor activities against MDA-MB-231 cells (IC50=3.90±0.04 μmol·L-1) and low toxicity to GES-1 cells (IC50>50 μmol·L-1). In the process of exploring the underlying mechanism of 3s, it was found that compound 3s could cause G2 phase arrest and apoptosis in MDA-MB-231 cells by overproduction of intracellular reactive oxygen species and collapse of mitochondrial membrane potential. The measured results were confirmed by western blot assay.

Key words: camphor-based thiosemicarbazone, anti-tumor activity, G2 phase arrest, reactive oxygen species (ROS), mitochondrial apoptosis pathway