Thirteen chemical constituents were isolated from the fruiting bodies of basidiomyctes Leccinum extremiorientale, and their structures were elucidated by spectral and physical-chemical data as: ergosta-7,22-dien-3/3,5ff,6/?-triol (1), ergosterol peroxide (2), ergosterol (3), ergosta-4,6,8(l4) ,22-tetraen-3-one (4), ergosta-5, 7- dien-3/3-ol (5), palmitic acid (6), (2S, 3S, AR, 2'R)-2-(2'- hydroxytetracosanoylamino) octadecane-1,3,4-triol (7), cerebroside B (8) , cerebroside D (9), uracil (10), inosine (11), adeosine (12) and D- allitol (13). The NMR data of compounds 8 and 9 were completely assigned by 2D-NMR techniques, including 'H^H COSY, HMBC and HMQC. Compounds 2, 7 and 8 at concentration of 100μg/mL showed selectively inhibitory activity against phospholipase A2 (PLA2) secreted from Crotalus adamenteus venom, but inactive against PLA2 of bee venom (Apis mellifcra). In addition, the methanol-chloroform soluble extract of this fungus also showed 67% antifeedant rate against Leucaninia separata and caused 55% mortality against Plutella xylostella after three-day application.

Key words: leccinum extremiorientale;CHEMICAL COMPOSITION;cerebrosides, PHOSPHOKINASE;INSECTICIDES