A new steroidal 14-hydroxy sprengerinin C (1) was isolated from the tuber of Ophiopogon japonicus (Thunb.) Ker-Gawl., along with three known compounds, sprengerinin C (2), ophiogenin-3-O-α-L- rhamnopyranosyl-(1→2)-β-D-glucopyranoside (3) and β-sitosterol-β-D-glucopyranoside (4). Their struc-tures were established on the basis of IR, HR-MS, 1D and 2D-NMR analyses, as well as by literature com-parison of spectroscopic data.

Key words: Ophiopogon japonicus, C27-steroidal glycoside, 14-hydroxy sprengerinin C, liliaceae