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Chin. J. Org. Chem. ›› 2008, Vol. 28 ›› Issue (03): 489-493. Previous Articles     Next Articles

Reports

一种含光亲和标记异戊烯侧链探针的合成与初步表征

李令东a,b，唐伟a,b，刘武军a,b，赵宗保*,a   

  1. (a中国科学院大连化学物理研究所生物技术部 大连 116023)
    (b中国科学院研究生院 北京 100049)
  • 收稿日期:2007-07-05 修回日期:2007-08-20 发布日期:2008-03-18
  • 通讯作者: 赵宗保

Synthesis and Activity of the Photoaffinity-Labeled Functional Probe Based on Prenyl Side-Chain

LI Ling-Donga,b,TANG Weia,b,LIU Wu-Juna,b,ZHAO Zong-Bao*,a   

  1. (a Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023)
    (b Graduate School of Chinese Academy of Sciences, Beijing 100049)
  • Received:2007-07-05 Revised:2007-08-20 Published:2008-03-18
  • Contact: ZHAO Zong-Bao

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A novel functional probe bearing both a benzophenone moiety and a biotin moiety was synthesized in 5 steps with an overall yield of 9%. Photoaffinity labeling and biotin blot experiment with the probe and the crude protein extracts from Saccharomyces cerevisiae AS 2.399 revealed that the synthetic probe was attached to a number of proteins, providing a basis for further chemical proteomics research.

Key words: functional probe, photoaffinity labeling, prenyl side-chain, chemical proteomics