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Chinese Journal of Organic Chemistry >

2013 , Vol. 33 >Issue 10: 2210 - 2215

DOI: https://doi.org/10.6023/cjoc201305013

Articles

A Reversible Fluorescence Enhancement Probe for Hg2+ Based on Rhodamine and Its Application in Live Cell Imaging

  • Chen Jiaxuan ,
  • Tian Yi ,
  • Xiang Qingxiang ,
  • Xiong Junru ,
  • Zhang Liqun ,
  • Li Yan
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  • Department of Chemistry, Leshan Normal University, Leshan 614000

Received date: 2013-05-10

  Revised date: 2013-05-31

  Online published: 2013-06-09

Supported by

Project supported by the Science and Technology Department (No. 2009JY0078), the Education Department of Sichuan Province (No. 11ZA154), and the Science and Technology Bureau of Leshan City (No. 2011SZD107).

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Abstract

A novel of Hg2+ fluorescence probe (RS) with rhodamine chromophore was synthesized, and its structure was characterized by 1H NMR, MS spectra and elemental analysis. The recognition properties of the probe RS with Hg2+ ions had been investigated in EtOH-HEPES buffer solution (V:V=1:1, pH=7.3) by the ultraviolet spectrophotometry and the fluorescence spectrophotometry. The results showed that the recognition is instantaneous and reversible. Upon the addition of Hg2+, the spirolactam ring of probe RS was opened and 1:1 ligand-mercury complex was formed. The fluorescence imaging experiments of Hg2+ in L929 living cells demonstrated that the probe RS could be used in biological systems.

Key words： rhodamine B; fluorescent probe; reversibility; Hg2+ ions; living cells

Cite this article

Chen Jiaxuan , Tian Yi , Xiang Qingxiang , Xiong Junru , Zhang Liqun , Li Yan . A Reversible Fluorescence Enhancement Probe for Hg2+ Based on Rhodamine and Its Application in Live Cell Imaging[J]. Chinese Journal of Organic Chemistry, 2013 , 33(10) : 2210 -2215 . DOI: 10.6023/cjoc201305013

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