Articles

Synthesis of a Reversible Fluorescence Probe for Hg2+ and Its Application in Living Cells

  • Zhou Jia ,
  • Yang Meipan ,
  • Meng Wenfei ,
  • Cheng Zhao ,
  • Yang Bingqin
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  • Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710069

Received date: 2014-03-05

  Revised date: 2014-03-28

  Online published: 2014-04-15

Supported by

Project supported by the National Natural Science Foundation of China (No. 21172178).

Abstract

A new rhodamine B derivative T1 has been rationally designed and synthesized facilely by the condensation reaction of rhodamine B hydrazide and o-phthaloyl chloride. The recognition properties of the target compound T1 with metal ions had been investigated by the fluorescence spectrophotometry. This probe exhibits several favorable features, including instantaneous and reversible recognition, high sensitivity and selectivity to mercury ions. When the concentration of Hg2+ was in the range of 4×10-8~9×10-8 mol/L, there was a good linearity between the fluorescence intensity and the concentration of Hg2+. The fluorescence imaging experiments of Hg2+ in HepG2 living cells demonstrated their valuable application in biological system.

Cite this article

Zhou Jia , Yang Meipan , Meng Wenfei , Cheng Zhao , Yang Bingqin . Synthesis of a Reversible Fluorescence Probe for Hg2+ and Its Application in Living Cells[J]. Chinese Journal of Organic Chemistry, 2014 , 34(8) : 1646 -1651 . DOI: 10.6023/cjoc201403009

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