Chinese Journal of Organic Chemistry >
Novel Fluorescence Probe for Fe3+and Application of Imaging in Living Cells
Received date: 2015-06-15
Revised date: 2015-07-11
Online published: 2015-08-26
Supported by
Project supported by the National Natural Science Foundation of China (No. 21172178).
A novel rhodamine B derivative R1 was synthesized facilely by the condensation reaction of rhodamine B, 2-aminothiophenol and benzyl chloride, and the structure of R1 was further characterized by IR, HRMS-ESI, 1H NMR, 13C NMR. Then, the recognition properties of compound R1 with metal ions had been investigated in water solution by the fluorescence spectrophotometry. The results showed that probe R1 demonstrated excellent specificity toward Fe3+with high speed and sensitivity, and the fluorescence intensity was not affected by the other common metal ions. Furthermore, there was a good linearity between the fluorescence intensity and the concentration of Fe3+. Importantly, fluorescence imaging experiments of Fe3+ in living cells demonstrated their valuable application.
Key words： fluorescence probe; rhodamine B; Fe3+; recognition; PC-12 living cells
Li Bo , Meng Wenfei , Yang Meipan , Su Na , Liu Xiaojing , Yang Bingqin . Novel Fluorescence Probe for Fe3+and Application of Imaging in Living Cells[J]. Chinese Journal of Organic Chemistry, 2015 , 35(12) : 2629 -2635 . DOI: 10.6023/cjoc201506017
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