Chinese Journal of Organic Chemistry >
Lysosome-Targeted Dual-Photon Nitroxyl Fluorescent Probe: Synthesis and Application in Living Cell Imaging
Received date: 2018-07-20
Revised date: 2018-08-27
Online published: 2018-09-18
Supported by
Project supported by the National Natural Science Foundation of China (Nos. 21572044, 21778013), the Natural Science Foundation of Hebei Provience (Nos. B2016201031, 2018201234), the Colleges and Universities Science Technology Research Project of Hebei Province (No. QN2017015) and the Science Tech-nology Research and Development Guidance Program Project of Baoding City (No. 16zg031).
Nitroxyl (HNO), which is the one-electron reduced and further protonated form of nitric oxide, plays important biological functions. A lysosome-targeted dual-photon HNO fluorescent probe (Lyso-HNO), which contains 4-(2-amino-ethyl)morpholine as lysosomal-targetable groups, 1,8-naphthalimide as two-photon fluorophore and triphenylphosphine as HNO reaction site, was synthesized and characterized. The recognition behaviors of Lyso-HNO to HNO were investigated. The results showed that Lyso-HNO exhibited good selectivity and sensitivity to HNO with fast response,. and the detection limit of Lyso-HNO to HNO was estimated to be 202 nmol·L-1. The probe can be applied to bioimaging exogenous lysosomal HNO by two-photon fluorescence confocal microscopy.
Key words： lysosome; dual-photon; nitroxyl; bioimaging
Wang Xiaofen , Wei Chao , Li Xueyan , Zheng Xueyang , Geng Xiaowei , Zhang Pingzhu , Li Xiaoliu . Lysosome-Targeted Dual-Photon Nitroxyl Fluorescent Probe: Synthesis and Application in Living Cell Imaging[J]. Chinese Journal of Organic Chemistry, 2019 , 39(2) : 469 -474 . DOI: 10.6023/cjoc201807032
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