Chinese Journal of Organic Chemistry >
A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells
Received date: 2018-08-28
Revised date: 2018-11-03
Online published: 2018-11-26
Supported by
Project supported by the Science and Technology Project of Henan Province (Nos. 182102311108, 182102310066), the Program for Science and Technology Innovation Talents in Universities of Henan Province (No. 15HASTIT002), and the National Natural Science Foundation of China (Nos. 21702178, U1304202).
A 3-hydroxyflavonoid esters HFBA was synthesized by the reaction of 2-hydroxyacetophenone with p-methyl benzaldehyde, and its structure was characterized by NMR and high resolution mass spectrometer. The recognition behaviors of HFBA to hydrazine were investigated and the results show that HFBA exhibits good selectivity and sensitivity to N2H4 with fast response and good anti-interference ability in DMSO/PBS buffer (pH=7.4). Furthermore, the detection limit of HFBA for hydrazine was estimated to be 0.11 μmol/L, and the probe HFBA was successfully applied for the imaging of hydrazine in living cells.
Key words： flavone; fluorescence; hydrazine; biological imaging
Ju Zhiyu , Shu Penghua , Xie Zhiyu , Jiang Yuqing , Tao Weijie , Xu Zhihong . A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells[J]. Chinese Journal of Organic Chemistry, 2019 , 39(3) : 697 -702 . DOI: 10.6023/cjoc201808035
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