Chinese Journal of Organic Chemistry >
Development of a New Carbon Monoxide Fluorescent Probe Based on Nitro Reduction and Its Bioimaging Research in Living Cells
Received date: 2020-09-07
Revised date: 2020-10-06
Online published: 2020-10-28
Supported by
National Natural Science Foundation of China(21672083); National Natural Science Foundation of China(21877048); National Natural Science Foundation of China(22077048); Startup Fund of Guangxi University(A3040051003); Natural Science Foundation of Guangxi Province(2019GXNSFBA245068)
Carbon monoxide (CO), as an important cell signaling molecule, is involved in important physiological and pathological functions in organism. A new fluorescence enhanced CO fluorescent probe NaLy-CO was developed by using naphthalimide dye as fluorescence platform and nitro group as the recognition site. The results of spectrum test in solution showed that the fluorescent probe NaLy-CO had good selectivity and sensitivity to CO. The fluorescent probe NaLy-CO was successfully used to detect exogenous and endogenous CO in living HeLa cells by using confocal fluorescence imaging technology. In addition, the colocation imaging experiments showed that the fluorescent probe NaLy-CO could detect CO in the lysosome of living cells.
Key words： carbon monoxide; fluorescent probe; bioimaging; nitro; naphthalimide
Enqing Chen , Yonghe Tang , Lei Wang , Jiangbo Ren , Weiying Lin . Development of a New Carbon Monoxide Fluorescent Probe Based on Nitro Reduction and Its Bioimaging Research in Living Cells[J]. Chinese Journal of Organic Chemistry, 2021 , 41(3) : 1200 -1206 . DOI: 10.6023/cjoc202009016
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