Chinese Journal of Organic Chemistry >
Development of an Ultrasensitive Mitochondria-Targeted Near Infrared Fluorescent Probe for SO2 and Its Imaging in Living Cells and Mice
Received date: 2020-12-29
Revised date: 2021-02-14
Online published: 2021-02-26
Supported by
Project supported by the NSFC(21672083); Project supported by the NSFC(21877048); Project supported by the NSFC(22077048); startup fund of Guangxi University(A3040051003); Guangxi Natural Science Foundation(2019GXNSFBA245068)
Sulfur dioxide (SO2) and its derivatives (sulfites, bisulfites, etc.), as important components of active sulfur species, are closely related to the physiological processes of living organisms. In addition, excessive intake of SO2 may cause irreparable damage to the respiratory system, such as lung cancer, tracheitis, asthma, etc. Near-infrared emission fluorescent probes have strong penetrating ability and can detect biomolecules in vivoeffectively. Therefore, in this paper, a mitochondrial targeted near-infrared SO2 fluorescent probe based on the Michael addition mechanism was designed. The solution spectroscopic experiments show that XA-SO2 has significant selectivity and sensitivity to SO2, and has the ability to rapidly detect SO2. It is worth noting that XA-SO2 has good membrane permeability and has successfully detected extracellular/endogenous levels. More importantly, XA-SO2 could be enriched effectively on the mitochondria of cells, which is promising for realizing the dynamic detection of SO2 in the mitochondria of living cells.
Key words： sulfur dioxide; fluorescent probe; bioimaging; Michael addition; mitochondria
Fang Li , Yonghe Tang , Rui Guo , Weiying Lin . Development of an Ultrasensitive Mitochondria-Targeted Near Infrared Fluorescent Probe for SO2 and Its Imaging in Living Cells and Mice[J]. Chinese Journal of Organic Chemistry, 2021 , 41(3) : 1108 -1116 . DOI: 10.6023/cjoc202012049
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