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Chinese Journal of Organic Chemistry >

2023 , Vol. 43 >Issue 7: 2561 - 2566

DOI: https://doi.org/10.6023/cjoc202301012

NOTES

In vivo Studies on the Biosynthetic Pathway of Aureonuclemycin and Identification of Key Metabolites

  • Fei Wang ,
  • Wenbing Jin ,
  • Xianfeng Hou ,
  • Gongli Tang ,
  • Haixue Pan
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  • aSchool of Chemistry and Materials Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024
    bShanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032
*E-mail: ;

Received date: 2022-11-11

  Revised date: 2023-02-24

  Online published: 2023-03-17

Supported by

National Natural Science Foundation of China(32070065)

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Abstract

On the basis of aureonuclemycin biosynthetic gene cluster obtained in the previous work, in-frame markerless deletion mutants of all genes were constructed, and the key genes that play a role in the biosynthesis of aureonuclemycin were identified. Two key aureonuclemycin-like compounds were successfully obtained from mutant and wild-type strains. According to the results of in vivo experiments, metabolite analysis and identification of key compounds, the possible biosynthetic pathway of aureonuclemycin was proposed.

Key words： nucleoside antibiotic; biosynthesis; aureonuclemycin

Cite this article

Fei Wang , Wenbing Jin , Xianfeng Hou , Gongli Tang , Haixue Pan . In vivo Studies on the Biosynthetic Pathway of Aureonuclemycin and Identification of Key Metabolites[J]. Chinese Journal of Organic Chemistry, 2023 , 43(7) : 2561 -2566 . DOI: 10.6023/cjoc202301012

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