Three new iridoid glycosides were isolated from Lamiophlomis rotata using silica gel, gel Sephadex LH-20 column chromatography, and preparative high-performance liquid chromatography. The structures of the new compounds were elucidated through analysis of spectroscopic evidence, including data obtained from high-resolution electrospray ionization mass spectrometry (HRESIMS) and nuclear magnetic resonance (NMR). The absolute configurations of the three compounds were confirmed by a combination of sugar hydrolysis, derivatization, and ECD calculations. The anti-inflammatory activities of compounds 1-3 were evaluated by measuring nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophages. All these new findings showed significantly inhibited LPS-induced NO production at the concentration of 7.5 μM.
Yan-Fang Ran
,
Li Huang
,
Jie Li
,
Li-Lian Zhao
,
Da-Le Guo
. Three Undescribed Iridoid Glycosides from Lamiophlomis rotata and Their Anti-inflammatory Activity[J]. Chinese Journal of Organic Chemistry, 0
: 202605008
.
DOI: 10.6023/cjoc202605008
[1] Lu R.; Wang L.; Yang R.; Chin. Wild Plant Resour,2026, 45, 117-123+131.
[2] Qiao F.; Lu Y.; Geng G.; Zhou L.; Chen Z.; Wang L.; Xie H.; Qiu Q.S.J Plant Physiol, 2023, 287, 154043.
[3] Wang M.; Yue G.; Chen Y.; Li K.; Jiang G.; Zhuoma Z.; Zhang Y.; Liu X.; Wang F.; Ren Q.; Li C.J Ethnopharmacol, 2026, 355, 120531.
[4] Geng X.; Wang Y.; Li H.; Song L.; Luo C.; Gu X.; Zhong H.; Chen H.; Chen X.; Wang J.; Pan Z.Chin Med, 2024, 19, 53.
[5] Jiang Q.; Hu M.; Li X.; Zhang Q.; Zhang X.; Jiang M. Int J Biol Macromol, 2025, 322, 146943.
[6] Xu Y.; Jiancuo S.; Luo X.; Ma Y.E.; Wu X.; Wu Z.; Yin H.; Zhang S.; Li W.; Sheng H.Biology (Basel), 2025, 14(11), 1554.
[7] Zhong L.; Li T.; Zhang J.; Zhang J.; Zhang J.; Li L.; Chen G.; Zhong S.; Gu R.BMC Plant Biol, 2025, 25, 941.
[8] Wang W.; Liu T.; Ding Y.; Liu X.; Qingji R.; Zhang, Y.; Zhang, Z.Drug Des Devel Ther, 2026, 20, 591283.
[9] Liu M.D.; Huang L.J.; Lei H.R.; Wang Y.J.; Hu Y.J.; Huo X.Y.; Li C.C.; Huang L.; Zhao L.L.; Zhang M.Z.; Gu Y.C.; Shyy M.; Sihota A.; Wang D.; Guo D.L.; Deng Y.Mar Life Sci & Technol, 2026, Doi: 10.1007/s42995-026-00358-5.
[10] Zhang C.Y.; Yang L.J.; Jiang L.S.; Mu Y.T.; Mao X.L.; Liu Q.; Deng F.; Guo D.L.Phytochem Anal, 2026, 37: 85-99.
[11] Roslund M.U.; Tahtinen P.; Niemitz M.; Sjoholm R.Carbohydr. Res. 2008, 343, 101-112.
[12] Zuo Y.; Pu Y.R.; Liu J.A.; Chen H. Y.; Chen Y.; Li X.L.; Wang Y.; Zhang T.T.; Cheng H.B.; Liu J.; Deng Y.; Cong Z.T.; Wang M.L.; Lu J.; Chen S.L.Acta Pharm. Sin. B 2025, DOI: 10.1016/j.apsb.2025.11.011.
[13] Zhang C.Z.; Chen Y.; Shi X.K.; Liu Y.; Zhou J.W.; Leng L.; Zhang T.T.; Liu S.J.; Liu J.; Wang T.B.; Zheng C.; Zhang C.T.; Deng Y.; Guo J.L.; Lu J.J. Controlled Release 2026, 391, 114654.
[14] Hu S.; Zhao L.L.; Li X.Y.; Ye Y.B.; Deng Y.; Huang L.J.; Guo D.L.Chin J Org Chem, 2026, 46: 300-303.
[15] Wu F.; Gu W.J.; Chen L.; Xia B.; Wang Y.; Huang L.J.; Wang K.; Gu Y.C.; Zhou Y.Phytochem Anal, 2026, 37: 273-286.
