淫羊藿炮制前后UPLC-PDA-MS 的指纹图谱研究

doi:10.6023/A1105271

化学学报 ›› 2012, Vol. 70 ›› Issue (05): 635-642 .DOI: 10.6023/A1105271 上一篇下一篇

研究论文

淫羊藿炮制前后UPLC-PDA-MS 的指纹图谱研究

朱粉霞^a, 赵永刚^b, 贾晓斌^a, 韦英杰^a, 张振海^a, 古丽娜尔·吾拉孜汗^a

a 江苏省中医药研究院国家中医药管理局中药释药系统重点研究室南京 210028;
b 江苏省环境监测中心南京 210036

投稿日期:2011-05-27 修回日期:2011-09-05 发布日期:2011-11-22
通讯作者: 贾晓斌 E-mail:xiaobinjia_nj@126.com
基金资助:
国家自然科学基金(No. 20973944)资助项目.

Study on Fingerprint of Crude and Processed Epimedium by UPLC-PDA-MS

Zhu Fenxia^a, Zhao Yonggang^b, Jia Xiaobin^a, Wei Yingjie^a, Zhang Zhenhai^a, Wulazihan Gulinaer^a

a Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028;
b Jiangsu Environmental Monitoring Center, Nanjing 210036

Received:2011-05-27 Revised:2011-09-05 Published:2011-11-22
Supported by:
Project was supported by the National Natural Science Foundation of China (No. 20973944).

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摘要/Abstract

建立了朝鲜淫羊藿和柔毛淫羊藿药材的超高效液相色谱(UPLC)指纹图谱分析方法, 实验结果表明, 两个品种的指纹图谱差别较大, 朝鲜淫羊藿标出35 个共有峰, 质谱鉴定了26 个峰, 柔毛淫羊藿标出13 个共有峰, 质谱鉴定了9个峰; 研究了朝鲜淫羊藿和柔毛淫羊藿炮制前后UPLC 指纹图谱的变化规律, 指纹图谱发生了五处变化. 该法快速、简便, 重现性好, 从整体上显示淫羊藿药材和炮制品的特征, 为淫羊藿的生品和炮制品的质量控制提供了有效手段.

关键词: 淫羊藿, UPLC-PDA-MS, 炮制, 指纹图谱

The fingerprint chromatograms of E. koreanum Nakai and E. pubescens Maxim were established by UPLC. The results showed that the fingerprints of two species were significantly different. There were 35 common peaks in crude drug of E. koreanum Nakai in which 26 peaks were identified, and 13 common peaks in crude drug of E. pubescens Maxim in which 9 peaks were identified, by MS respectively. Additionally, there were 5 differences in fingerprint between crude drug and processed product. This method with desirable rapidness, simplity and repeatability can be used for the quality evaluation of crude and processed Epimedium.

Key words: Epimedium, UPLC-PDA-MS, processed, fingerprint

引用此文

朱粉霞, 赵永刚, 贾晓斌, 韦英杰, 张振海, 古丽娜尔·吾拉孜汗. 淫羊藿炮制前后UPLC-PDA-MS 的指纹图谱研究[J]. 化学学报, 2012, 70(05): 635-642 .

Zhu Fenxia, Zhao Yonggang, Jia Xiaobin, Wei Yingjie, Zhang Zhenhai, Wulazihan Gulinaer. Study on Fingerprint of Crude and Processed Epimedium by UPLC-PDA-MS[J]. Acta Chimica Sinica, 2012, 70(05): 635-642 .

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淫羊藿炮制前后UPLC-PDA-MS 的指纹图谱研究

Study on Fingerprint of Crude and Processed Epimedium by UPLC-PDA-MS

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