水相合成近红外CdTe量子点用于体液中硫醇的检测

doi:10.6023/A13090931

摘要/Abstract

通过降低反应物中L-半胱氨酸(L-Cys)的比例，在水相快速合成了近红外CdTe量子点，使之对巯基化合物产生荧光响应. 并以此构建了一种基于表面配体缺失的CdTe近红外荧光量子点的巯基探针，为生物样品中的硫醇检测提供简便经济、高灵敏度和高选择性的新方法. 在其它多种氨基酸和生物液体中主要离子、分子共存的情况下，我们所制备的近红外量子点对L-Cys、同型半胱氨酸(Hcy)和谷胱甘肽(GSH)的荧光检测中显示了良好的选择性和灵敏度. 在血清和细胞提取液中，加标5.0 μmol·L^-1硫醇的回收率均在90%～109%范围内. 该方法对L-Cys，Hcy和GSH的检出限(3s)分别为43，46和63 nmol·L^-1.

关键词: 量子点, 水相合成, 近红外荧光, 检测硫醇

As well known that conventional aqueous synthesis of the near-infrared (NIR) CdTe quantum dots (QDs) using thiol ligands as capping reagents is usually very time-consuming. To overcome this defect and prepare NIR CdTe QDs, we present a fast and facile route in aqoueous phase under atmospheric pressure using L-cysteine (L-Cys) as capping reagents. The influences of various experimental conditions on the growth rate and luminescent properties of the obtained CdTe QDs have been systematically investigated, including Te-to-Cd ratio, L-Cys-to-Cd ratio and pH value. The experiment results suggested that lower ratio of Te:Cd or L-Cys:Cd and high pH value would markedly shortened the reaction time. Furthermore, the obtained QDs were used as a kind of NIR fluorescent probes for thiol detection in biological fluids. The change in the fluorescence intensity of the NIR CdTe QDs in the presence of 5.0 μmol·L^-1 homocysteine (Hcy), L-Cys or glutathione (GSH) with different interaction time was measured. The effect of pH on the enhanced fluorescence intensity of the NIR CdTe QDs (10 mg·L^-1) at 5.0 μmol·L^-1 Hcy, L-Cys or GSH and the fluorescence responses of NIR CdTe QDs to 20 essential amino acids (5.0 mmol·L^-1 for L-Cys, 5.0 mmol·L^-1 for the other 19 amino acids) in pH 7.0 PBS buffer was investigated. The probe offered good sensitivity and selectivity for detecting L-Cys, Hcy and GSH in the presence of 20 other amino acids, main relevant metal ions, and some other molecules in biological fluids. The recovery of spiked 5.0 mmol·L^-1 thiols in human serum and cell extracts ranged from 90% to 109%. The precision for 11 replicate measurements of the thiols at 5.0 mmol·L^-1 is in the range of 2.4%～3.3%. The detection limits (3s) for L-Cys, Hcy and GSH are 43, 46 and 63 nmol·L^-1, respectively. For real sample measurement, four serum samples and cell extract sample from two cancer cell lines (Hela and HepG2) were chosen and the analytical results were comparable with HPLC assay.

Key words: quantum dots, aqueous synthesis, near-infrared fluorescence, biothiol detection

引用此文

崔晓腾, 吕玉洋, 刘颖, 吴伯岳. 水相合成近红外CdTe量子点用于体液中硫醇的检测[J]. 化学学报, 2014, 72(1): 75-82.

Cui Xiaoteng, Lv Yuyang, Liu Ying, Wu Boyue. Aqueous Synthesis of Near-Infrared CdTe Quantum Dots for Biothiols Detection in Biological Fluids[J]. Acta Chimica Sinica, 2014, 72(1): 75-82.

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