### 三种金属硫蛋白聚合物静电效应的研究

1. 北京大学化学与分子工程学院;北京大学生命科学学院;中国科学院生物物理研 究所.北京(100101)
• 发布日期:2000-03-15

### Electrostatic interactions in three types of metallothioneins aggregates

Hou Tingjun;Li Youyong;Xu Xiaojie;An Yu;Ru Binggen;Bi Ruchang

1. Inst of Biophysics, CAS.Beijing(100101)
• Published:2000-03-15

The electrostatic potential calculations were carried out on three types of metallothioneins. The structure of MT-II in rat liver was obtained directly from protein data bank, while other two structures of MT-I and MT-II in rabbit were constructed by homology modeling. The electrostatic potential was calculated by solving the Poisson- Boltzmanne equation using the finite difference method. In each dimer, two monomers exhibited apparent electrostatic complementarity in buffers of pH5.6-8.5. But when pH was increased to 10.6, the electrostatic complementarity would be reduced singnificantly. For all trimers, the electrostatic interactions between the monomer and dimer were unfavorable. Moreover, the electrostatic repulsion would be significantly enhanced when the pH value was changed to 10.6.