关键词: 吸收光谱法, 脱氧核糖核酸, 有机锗化合物, 嵌入反应, 抗癌药, 药物设计

The interaction between Ge-132 and DNA was investigated using absorption spectra, DNA alkaline denaturation and fluorescence spectra. Based on the measurements of A~2~6~0 in different pH media, the alkaline denaturation curves of CT DNA in the absence of and in the presence of Ge-132 were obtained. The results showed that the alkaline denaturation for Ge-132-DNA complex occurred at a higher pH than for DNA alone and the hyperchromicity of Ge-132-DNA complex was smaller than that of DNA, which suggested the intercalation mechanism of Ge-132 into DNA bases. All the exprimental results indicated that the intercalative binding was the major mode for the interaction of Ge-132 with DNA.

Key words: ABSORPTION SPECTROMETRY, DEOXYRIBONUCLEIC ACID, ORGANO GERMANIUM, INTERCALATION REACTION, ANTICARCINOGEN