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化学学报
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化学学报 ›› 2004, Vol. 62 ›› Issue (10): 1003-1006. 上一篇    

研究简报

尼古丁是脂肪酶的反竞争抑止剂的实验研究

林琳1, 陈锡侨2, 赵福群1, 唐应武1, 张复实1   

  1. 1. 清华大学化学系, 有机光电子与分子工程教育部重点实验室, 北京, 100084;
    2. 澳门大学科技学院, 澳门, 邮政信箱3001号
  • 投稿日期:2003-08-25 修回日期:2004-03-15 发布日期:2014-02-17
  • 通讯作者: 张复实,E-mail:zhangfs@tsinghua.edu.cn E-mail:zhangfs@tsinghua.edu.cn
  • 基金资助:
    澳门大学研究基金(RG013/02-03W/CSK/FST)资助项目.

Study of Nicotine Acting as an Uncompetitive Inhibitor of Lipase

LIN Lin1, CHAN Shek-Kiu2, ZHAO Fu-Qun1, TANG Ying-Wu1, ZHANG Fu-Shi1   

  1. 1. Key Lab of Organic Optoelectronic & Molecular Engineering, Ministry of Education Department of Chemistry, Tsinghua University, Beijing 100084;
    2. Department of Science and Technology, University of Macau, Macau
  • Received:2003-08-25 Revised:2004-03-15 Published:2014-02-17

PDF

324

被引次数

21

希望明确尼古丁对脂肪酶活性是否有抑止作用,用于解释医学已有统计数据:吸烟的人体重普遍较轻,而戒烟后体重会有回升.用三油酸甘油酯为底物,在加入尼古丁前后,检测脂肪酶活性变化.结果表明:脂肪酶在人体外仍有活性,而且尼古丁是脂肪酶反竞争性抑止剂.在脂肪酶浓度为7 mg/mL时,抑止程度达50%.

关键词: 脂肪酶, 尼古丁, 三油酸甘油酯, 油酸

The aim of the study is to determine the role of nicotine as a lipase inhibitor in fat and fatty acid metabolism and to explain the clinic phenomenon: smokers weigh less than age-matched nonsmokers, and most of smokers gain weight after smoking cessation. Lipase activity was measured in the presence or absence of nicotine using olein as substrate. Lipase is active in vitro. Nicotine is demonstrated to be an uncompetitive inhibitor of lipase at biologically reachable concentration. It is shown that the activity of lipase was reduced to 50% at lipase concentration of 7 mg/mL.

Key words: lipase, nicotine, olein, oleic acid