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化学学报
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化学学报 ›› 2005, Vol. 63 ›› Issue (12): 1147-1150. 上一篇    下一篇

研究简报

膜片钳法研究镁离子对萝卜液泡膜SV通道的影响

张朝峰，杨频*，薛绍武   

  1. (山西大学分子科学研究所 化学生物学与分子工程教育部重点实验室 太原 030006)
  • 投稿日期:2004-12-22 修回日期:2005-02-28 发布日期:2010-12-10
  • 通讯作者: 杨频

Study of Magnesium Effects on Slow Vacuolar Ion Channel in Radish by Patch-Clamp Technique

ZHANG Chao-Feng, YANG Pin*, XUE Shao-Wu   

  1. (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006)
  • Received:2004-12-22 Revised:2005-02-28 Published:2010-12-10
  • Contact: YANG Pin

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977

被引次数

11 | 2

用膜片钳全液泡记录方式研究了Mg2＋对萝卜(Raphanus sativus L.)液泡膜上SV通道电流的影响. 结果表明: 用EGTA配位Ca2＋后, 胞质Mg2＋不能够代替Ca2＋来激活SV通道; 外液中不同浓度的Mg2＋对通道电流有抑制作用, 并且呈一定的浓度依赖性, 用Hill方程拟合浓度依赖性曲线, 得抑制常数Ki＝(1.94±0.11) mmol/L, 而内液中的Mg2＋不影响通道电流. 这一结果为进一步研究镁对植物生理活动的影响从通道水平提供了重要依据.

关键词: SV通道, 胞质镁, 膜片钳, 液泡

The effects of magnesium cation on slow vacuolar (SV) channel currents in radish were studied using the whole-vacuole patch-clamp technique. In the presence of Ca2＋ buffer EGTA, cytosolic Mg2＋could not activate SV currents instead of cytosolic Ca2＋. Mg2＋added to bath solution caused a decrease of SV currents in a concentration-dependent manner. The concentration response curve could be fitted to a Hill equation with a Ki of (1.94±0.11) mmol/L. Varying the luminal Mg2＋concentration had no effect on SV currents. These results will provide an important basis at channel level for the further research of the effects of magnesium on physiological activities of plants.

Key words: SV channel, cytosolic Mg2＋, patch-clamp, vacuole