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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (3): 229-234. 上一篇    下一篇

研究论文

毛细管电泳微芯片在临床尿蛋白检测中的应用研究

庄贵生1,2，刘菁1,2，贾春平1，金庆辉1，王惠民3, 杨梦苏*,1，赵建龙1   

  1. (1中国科学院上海微系统与信息技术研究所 上海 200050)
    (2中国科学院研究生院 北京 100049)
    (3南通大学附属医院 南通 226001)
  • 投稿日期:2005-04-11 修回日期:2005-10-18 发布日期:2006-02-14
  • 通讯作者: 杨梦苏

Capillary Electrophoresis Microchip Assay of Clinical Urinary Proteins

ZHUANG Gui-Sheng1,2, LIU Jing1,2, JIA Chun-Ping1, JIN Qing-Hui1, WANG Hui-Min3, YANG Meng-Su*,1, ZHAO Jian-Long1   

  1. (1 Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050)
    (2 Graduate School of the Chinese Academy of Sciences, Beijing 100049)
    (3 Affiliated Hospital of Nantong University, Nantong 226001)
  • Received:2005-04-11 Revised:2005-10-18 Published:2006-02-14
  • Contact: YANG Meng-Su

PDF

1080

被引次数

13

用微芯片毛细管电泳法对临床患者尿蛋白进行了分离, 初步探讨了用于判断肾损伤的应用前景. 以pH 10.3, 75 mmol•L－1的硼酸盐缓冲液作为芯片电泳缓冲体系, 利用蛋白质的紫外吸收特性, 在210 nm波段检测吸光度并进行信号收集和分析. 研究两种添加剂对提高尿蛋白分离效率的影响, 分析了肾病综合症、妊娠高血压症、风湿性心脏病和多发性骨髓瘤等患者尿样本, 并与美国Helena电泳系统分析结果对比, 得到了较一致的结果.

关键词: 毛细管电泳微芯片, 紫外检测, 临床诊断, 尿蛋白

The separation and detection of urinary proteins have been conducted by microchip-based capillary electrophoresis (MCE), and application foreground of MCE in clinical diagnosis of various states of disease was discussed. Microchip-based capillary electrophoresis of urinary proteins could offer several advantages over standard urinary protein assay methods such as SDS-PAGE and conventional capillary electrophoresis, including the decrease of sample consumption and analysis time. The microchip used in these studies was designed to realize on-chip separation of urinary proteins with UV detection. The characteristic absorbency near 210 nm of proteins was adopted under the condition: the electrophoretic buffer at 75 mmol•L－1 borate with 0.5 mol•L－1 lactate at pH 10.5. Urine samples from four clinical cases were selected as target for microchip-based urinary protein assays, and the results were consistent with those of commercial Helena Rapid Electrophoreses system.

Key words: capillary electrophoresis microchip, UV detection, clinical diagnosis, urinary protein