化学学报 ›› 2008, Vol. 66 ›› Issue (15): 1845-1849. 上一篇    

研究简报

手性微柱高效液相色谱用于对映体的分离

朱智甲a 王倩倩a 康经武*,b

  

  1. (a东华大学化学化工与生物工程学院 上海 201620)
    (b中国科学院上海有机化学研究所 上海 200032)

  • 收稿日期:2008-03-20 修回日期:2008-06-24 出版日期:2008-08-14 发布日期:2008-08-14
  • 通讯作者: 康经武

Enantioseparation with A Chiral Micro-HPLC System

ZHU, Zhi-Jia a WANG, Qian-Qian a KANG, Jing-Wu *,b   

  1. (a College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620)
    (b Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032)
  • Received:2008-03-20 Revised:2008-06-24 Online:2008-08-14 Published:2008-08-14
  • Contact: KANG, Jing-Wu

报道了实验室自制的手性微柱高效液相色谱系统和纤维素类手性填料的制备方法, 以及用于对映体分离的应用研究. 研究了手性微柱的装填技术并考察了影响填料性能的关键因素. 优化了流动相的组成和流速对对映体拆分的影响. 成功地分离了5种对映体. 结果表明, 该系统操作简便、分离度高, 而且流动相消耗低, 分析速度快. 该系统可以用于实验室手性分离分析.

关键词: 微柱高效液相色谱, 纤维素三(3,5-二甲基苯基氨基甲酸酯), 对映体分离

The present paper describes the protocol for the preparation of a home made chiral micro-HPLC system and the chiral packing material with cellulose-based chiral stationary phase. The column packing technique was investigated and the key factors which may influence the performance of the chiral separation system were evaluated with several chiral compounds. The composition of the mobile phase and its flow rate were optimized. Finally, five enantiomers were successfully separated. It was illustrated that the system was characterized by simplicity, high resolution, low consumption of the mobile phase and high speed. The system cauld be easily prepared at the laboratory for enantioseparation.

Key words: micro-column HPLC, cellulose tris(3,5-dimethylphenylcarbamate), enantioseparation