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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (21): 2439-2444. 上一篇    下一篇

研究论文

乌头碱在家兔肠道内代谢产物的LC/ESI-MSn研究

随志刚a,d    姜雅秋b    刘志强c    梁 峰a    闫 峻a    刘忠英*,a   

  1. (a吉林大学药学院 长春 130021) (b吉林大学第一医院 长春 130021) (c中国科学院长春应用化学研究所 长春 130022) (d吉林大学白求恩医学院 长春 130021)
  • 投稿日期:2009-02-12 修回日期:2009-06-16 发布日期:2009-07-01
  • 通讯作者: 刘志强 E-mail:liuzq@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(30873360)资助

Study on Metabolites of Aconitine in Rabbit Intestines by Using LC/ESI-MSn

Sui, Zhigang a,d   Jiang, Yaqiub    Liu, Zhiqiangc    Liang, Fenga    Yan, Juna    Liu, Zhongying*,a   

  1. (a College of Pharmaceutical, Jilin University, Changchun 130021) (b The First Hospital of Jilin University, Changchun 130021) (c Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022) (d Norman Bethune College of Medicine, Jilin University, Changchun 130021)
  • Received:2009-02-12 Revised:2009-06-16 Published:2009-07-01

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采用LC/ESI-MSn的方法对家兔肠道内的乌头碱代谢产物进行研究. 经与空白组比较发现, 给药后家兔小肠内容物中新增加6个化合物峰(M1～M6), 盲肠内容物中新增加5个化合物峰(M2～M6), 粪便中新增加2个化合物峰(M3, M4). 分别测定各化合物的准分子离子及各级串联碎片离子, 并与标准品的质谱断裂规律进行比较, 同时参考文献, 推断肠道内化合物M1为16-O-去甲基-8-O-去乙酰基乌头碱, M2为8-O-去乙酰基乌头碱, M3为16-O-去甲基乌头碱, M4为乌头碱(AC), M5为去氧乌头碱, M6为印乌头碱.

关键词: 乌头碱, 代谢产物, 液相色谱-电喷雾电离-多级串联质谱法

LC/ESI-MSn was used to separate and determine the major metabolites of aconitine in intestines of rabbit. Compared with the control samples, six compounds (M1～M6) were found in small intestine, five compounds (M2～M6) in caecum and two compounds (M3, M4) in feces. On the bases of MSn fragmentation of standard and references, they were deduced as 16-O-demethyl-8-O-deacetylaconitine (M1), 8-O-deacetylaconitine (M2), 16-O-demethylaconitne (M3), aconitine (M4), deoxyaconitine (M5) and indaconitine (M6).

Key words: aconitine (AC), metabolites, LC/ESI-MSn