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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (3): 185-190.    下一篇

研究论文

氢化松香酸钠与明胶蛋白质相互作用的荧光光谱法研究

唐世华* 李景权 雷福厚

  

  1. (广西民族大学化学与生态工程学院 南宁 530006)

  • 投稿日期:2008-07-15 修回日期:2008-08-19 发布日期:2009-02-14
  • 通讯作者: 唐世华

Fluorescence Spectral Study on Interaction of Hydrogenated Sodium Rosinate with Gelatin

Tang, Shihua* Li, Jingquan Lei, Fuhou   

  1. (College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006)
  • Received:2008-07-15 Revised:2008-08-19 Published:2009-02-14
  • Contact: Tang, Shihua

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被引次数

24 | 5

在pH＝10.80及不同温度下，, 利用常规荧光法、同步荧光法、共振光散射和紫外-可见光谱研究了氢化松香酸钠(HSR)与明胶的相互作用. 结果表明HSR与明胶有一个结合位点，, 聚集体的形成引起明胶内源荧光增强.. 据此建立了体系荧光增强与HSR浓度的线性关系式，, 计算了不同温度下反应的结合常数及对应温度下结合反应的热力学参数.. 二者之间主要靠疏水作用力结合..

关键词: 氢化松香酸钠, 明胶, 荧光增强公式, 同步荧光, 共振光散射

The interaction between hydrogenated sodium rosinate (HSR) and gelatin in aqueous solution was investigated by fluorescence spectroscopy, synchronous fluorescence spectroscopy, resonance light scattering (RLS) and UV-Vis spectra at pH 10..80 and different temperatures.. The experimental result showed that HSR and gelatin formed a 1∶1 aggregate.. The equation for calculating the binding constant in of HSR for to gelatin was introduced based on the relationships between the fluorescence enhancement of gelatin and the concentration of HSR.. According to the fluorescence enhancement equation, the binding constants of the aggregate at different temperatures and the thermodynamic parameters at corresponding temperatures were obtained.. It was suggested that theThe binding force between HSR and gelatin wasshould be suggested to be from hydrophobic interaction..

Key words: hydrogenated sodium rosinate, gelatin, fluorescence enhancement equation, synchronous fluorescence, resonance light scattering