SIOC English
化学学报
高级检索

化学学报 ›› 2010, Vol. 68 ›› Issue (13): 1303-1307. 上一篇    下一篇

研究论文

基于分子信标定量检测肿瘤细胞p21 mRNA表达变化

刘斌1,2,3，羊小海1,3，王柯敏*,1,2,3，谭蔚泓1,2,3   

  1. (1化学生物传感与计量学国家重点实验室 湖南大学化学化工学院 长沙 410082)
    (2湖南大学生命科学与技术研究院 长沙 410082)
    ( 3生物纳米与分子工程湖南省重点实验室 长沙 410082)
  • 投稿日期:2009-11-06 修回日期:2010-01-29 发布日期:2010-03-04
  • 通讯作者: 王柯敏 E-mail:kmwang@hnu.cn

Quantitative Detection of p21 mRNA in Tumor Cells Based on Molecular Beacon

Liu Bin1,2,3, Yang Xiaohai 1,3 Wang Kemin*,1,2,3 Tan Weihong 1,2,3   

  1. (1 State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082)
    (2 Life Science and Biotechnology Institute, Hunan University, Changsha 410082)
    (3 Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082)
  • Received:2009-11-06 Revised:2010-01-29 Published:2010-03-04

PDF

1232

被引次数

4

根据p21基因序列设计合成特异性检测p21 mRNA的分子信标, 发展了一种体外快速、定量检测p21 mRNA的新方法, 将其用于肿瘤细胞p21 mRNA表达水平的检测. 结果发现: 抑制p53表达引起CNE2细胞中p21 mRNA表达水平降低, 转染ING1诱导MCF-7细胞中p21 mRNA表达上调, 5-氟尿嘧啶处理MCF-7细胞后p21 mRNA表达水平呈浓度和时间相关性变化. 这种特异性强、操作快速、简便的新方法有望广泛用于各类样品中p21 mRNA表达水平检测.

关键词: 分子信标, p21, RT-PCR, 定量检测

A molecular beacon, which was designed and synthesized according to the p21 gene sequence, was applied to develop a new method for quantitative detection of p21 mRNA in cellular total RNA. The results showed that p21 mRNA is inhibited by silencing p53 in CNE2 cells while it is upregulated in MCF-7 cells by ING1 transfection, the influence of 5-FU treatment on p21 mRNA of MCF-7 cells is time and concentration-dependent manner. These results demonstrated that the new method with the advantage of high specificity, fast speed and simplicity can be widely applied for p21 mRNA detection in different samples.

Key words: molecular beacon, p21, RT-PCR, quantitative detection