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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (01): 77-83. 上一篇    下一篇

研究论文

表面解吸常压化学电离质谱快速分析六味地黄丸

越皓1，肖治国2，王恩鹏1，陈焕文3，张兴磊3，贾滨3，刘淑莹*,1   

  1. (1长春中医药大学吉林省人参科学研究院 长春 130117)
    (2长春大学计算机科学技术学院 长春 130022)
    (3东华理工大学应用化学系 抚州 344000)
  • 投稿日期:2010-03-19 修回日期:2010-09-04 发布日期:2010-09-18
  • 通讯作者: 刘淑莹 E-mail:mslab@ciac.jl.cn
  • 基金资助:

    吉林省科技发展计划;吉林省重大科技发展计划

Rapid Analysis of Liuwei Dihuang Pills Using Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry

Yue Hao1, Xiao Zhiguo2, Wang Enpeng 1, Chen Huanwen3 Zhang Xinglei3 Jia Bin3 Liu Shuying*,1   

  1. (1 Changchun University of Traditional Chinese Medicine, Jilin Ginseng Academy, Changchun, 130117 )
    (2 Chanchun University, College of Computer Science and Technology, Changchun, 130022)
    (3 East China Institute of Technology, Department of Applied Chemistry, Fuzhou, 344000)
  • Received:2010-03-19 Revised:2010-09-04 Published:2010-09-18

PDF

1175

被引次数

18

采用新型表面解吸常压化学电离(Surface Desorption Atmospheric Pressure Chemical Ionization, SDAPCI)质谱法, 在敞开环境下, 对潮湿的空气进行电晕放电产生试剂离子, 进而在六味地黄丸表面发生解吸电离过程, 在无需复杂预处理的前提下对六味地黄丸中的待测物进行离子化, 从而获得了六味地黄丸在正负离子模式下的化学指纹图谱, 并利用主成分分析法对质谱指纹数据进行处理, 可对6个厂家生产的多个批次产品进行较好的区分. 结果表明, SDAPCI-MS技术能够快速测定六味地黄丸的剂型和生产厂家信息, 并能够对目标组分做多级串联质谱鉴定, 发现痕量目标组分. 研究方法可望应用于中成药药品生产质量控制和成品检测等领域.

关键词: 表面解吸常压化学电离, 六味地黄丸, 指纹图谱, 快速分析, 主成分分析

A surface desorption atomospheric pressure chemical ionization mass spectrometry (SDAPCI-MS) method was developed to obtain the fingerprint of Liuwei Dihuang pills (LDP) with minimal sample pre-treatment. In the open environment, humid air was corona discharged to produce reagent ions for desorption ionization of the analytes on the surface of the LDPs. Then the analyte ions were guided into the ion trap mass analyzer of the LTQ instrument for mass analysis. Identification of the components of interests such as gallic acid, paeonol and ursolic acid in Liuwei Dihuang pills were demonstrated by tandem mass spectrometry (MS/MS). Principal component analysis (PCA) of the mass spectral fingerprint data was used to differentiate the samples from four manufacturers. The results show that this method is a useful analytical tool for quality control in pharmaceutical industry, particularly for the traditional Chinese medicine production.

Key words: surface desorption atomospheric pressure chemical ionization mass spectrometry, Liuwei Dihuang pill, fingerprint, rapid analysis, principal component analysis (PCA)