化学学报 ›› 2006, Vol. 64 ›› Issue (15): 1553-1558. 上一篇    下一篇

研究论文

基于一次性薄层色谱电极高选择电化学发光分析法测定Ni(II)的研究

李桂新1, 郑行望*,1, 熊海涛1,2, 章竹君1   

  1. (1陕西师范大学化学与材料科学学院 西安 710062)
    (2陕西理工学院化学系 汉中 723001)
  • 收稿日期:2005-12-14 修回日期:2006-01-23 出版日期:2006-08-14 发布日期:2006-08-14
  • 通讯作者: 郑行望

Highly Selective Electrogenerated Chemiluminescence Method for Determination of Ni(II) with the Disposable Micro-Thin-Layer Chromatography Electrode

LI Gui-Xin1, ZHENG Xing-Wang*,1, XIONG Hai-Tao1,2, ZHANG Zhu-Jun1   

  1. (1 College of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062)
    (2 Department of Chemistry, Shaanxi University of Technology, Hanzhong 723001)
  • Received:2005-12-14 Revised:2006-01-23 Online:2006-08-14 Published:2006-08-14
  • Contact: ZHENG Xing-Wang

基于一种新的电极制作方法, 研制了具有薄层色谱分离功能的一次性薄层色谱电极. 结合该电极的分离、保留分析物于电极表面一定空间区域的色谱分离能力与电极表面电化学发光信号的空间分辨能力, 实现了分析物的高效分离与原位高灵敏度电化学发光(ECL)检测, 建立了电化学发光分析方法与薄层色谱分离方法联用的新技术. 并以Ni2+离子为代表探讨了这一方法的可行性和分析特性. 在最佳的实验条件下, 该方法测定Ni2+离子的线性范围为5.0× 10-9~5.0×10-6 g/mL, 检出限为1.5×10-9 g/mL, 相对标准偏差为2.8% (c=1.0×10-6 g/mL, n=11).

关键词: 电化学发光, 一次性薄层色谱电极, 鲁米诺, 镍离子

Based on a new method of electrode preparation, a novel disposable micro-thin-layer chromatography electrode with the separating function of thin-layer chromatography (TLC) was designed. Combined the ability of separating, retaining the analyte in a certain space of the electrode with the high spatial-resolved ability of electrogenerated chemiluminescence (ECL) signal on the surface of electrode, this method attained highly effective and in situ separation, highly sensitive electrogenerated chemiluminescence detection to the analyte, and a novel method is presented for the coupling of thin-layer chromatography with the ECL. The feasibility and analytical performance of this method was investigated by choosing Ni2+ as the analyte. Under the optimal experiment conditions, the linear range of the proposed ECL method for Ni2+was 5.0×10-9 to 5.0×10-6 g/mL with a 1.5×10-9 g/mL detection limit, and the relative standard deviation was 2.8% (c=1.0×10-6 g/mL, n=11).

Key words: electrogenerated chemiluminescence, disposable micro-thin-layer chromatography electrode, luminol, nickel ion