化学学报 ›› 2000, Vol. 58 ›› Issue (7): 856-861. 上一篇    下一篇

研究论文

磷脂酰甘油与缺钙光系统Ⅱ的相互作用

阳振乐;李良璧;许亦农;匡廷云   

  1. 中国科学院植物研究所.北京(100044);中国科学院光合作用基础研究开放实验 室
  • 发布日期:2000-07-15

Interaction of phosphatidylglycerol with Ca^2^+ - depleted photosystemⅡ

Yang Zhenle;Li Liangbi;Xu Yinong;Kuang Tingyun   

  1. Inst of Botany, CAS.Beijing(100044)
  • Published:2000-07-15

对磷脂酰甘油(PG)对缺钙光系统Ⅱ(d~C~aPSⅡ)放氧活性的影响及其作用机制进行了研究。结果表明,PG除能促进PSⅡ的放氧活性以外,还对PSⅡ系统表现出新的作用,即PG可以使PSⅡ颗粒因缺钙而受抑制的放氧活性得到恢复;外加Ca^2^+可使PG表现出对d~C~aPSⅡ放氧活性的更大促进作用,且随Ca^2^+浓度的增加,促进作用也越显著。PG的作用就在于恢复蛋白的正常结构,而PG与Ca^2^+形成的配合物可以优化这种结构而使缺钙PSⅡ颗粒的放氧活性得到增强。

关键词: 磷脂酰甘油, 缺钙光系统II, 钙离子, 付里叶变换, 红外分光光度法

The effect of anionic phosphatidylglycerol (PG) on oxygen evolution in a photosystem Ⅱ(PSⅡ) particle depleted of Ca^2^+ designated as d~C~aPSⅡ in this work and the mechanism of their interaction were investigated. The major finding is the observation of a new role of PG in the PSⅡ function, that is, PG restores nearly the lost oxygen evolution in d~C~aPSⅡ particle owing to Ca^2^+ deficiency to the level in intact PSⅡ in which the same effect was achieved in our previous study. furthermore, there is a stimulation of oxygen- evolving activity in the d~C~aPSⅡ complexed with PG in the presence of exogenously added Ca^2^+, which PG enhances increasingly oxygen evolution with increasing Ca^2^+ concentration. It revealed that PG- induced oxygen evolution recovery results from resumption of normal structure in protein by PG effect, whereas the enhancement of oxygen evolution in d~C~aPSⅡ~PG complex subject to Ca^2^+ is ascribed to the optimization of such a structure due to coordination complex formation of Ca^2^+ ions with PG.

Key words: PHOSPHATIDYL GLYCEROL, CALCIUM ION, FOURIER TRANSFORM, INFRARED SPECTROPHOTOMETRY

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